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Updated: Jul 28, 2025
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
Published on: September 28, 2011
Complete Genome Sequence of Pseudomonas aeruginosa Phage UF_RH6, Isolated from Human Lung
Abdolrazagh Hashemi Shahraki1, Majid Vahed1, Shahla Masood2
1Division of Pulmonary, Critical Care, and Sleep Medicine, College of Medicine-Jacksonville, University of Florida, Jacksonville, Florida, USA.
Abstract:
We report the genome sequence of a lytic phage named UF_RH6, which infects Pseudomonas aeruginosa. This phage was isolated from a respiratory secretion sample from a patient with pulmonary P. aeruginosa. UF_RH6 belongs to the family Caudoviricetes and the genus Samunavirus. Its genome is 94,715 bp in length and encodes 130 proteins.
Insights
Researchers sequenced the genome of UF_RH6, a lytic phage targeting Pseudomonas aeruginosa. This phage was isolated from a patient with lung infections, offering potential insights into phage therapy.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Pseudomonas aeruginosa is an opportunistic pathogen causing severe infections, particularly in immunocompromised individuals.
- Phage therapy presents a promising alternative or adjunct to antibiotics for treating bacterial infections.
- Lytic phages, which kill host bacteria, are of significant interest for therapeutic applications.
Purpose of the Study:
- To determine the complete genome sequence of the lytic phage UF_RH6.
- To characterize UF_RH6, a phage isolated from a clinical sample of Pseudomonas aeruginosa.
- To provide genomic data that can inform future research on phage-bacteriotherapy.
Main Methods:
- Isolation and purification of the lytic phage UF_RH6 from a respiratory secretion sample.
- Whole-genome sequencing of the UF_RH6 phage.
- Bioinformatic analysis to identify encoded proteins and phylogenetic placement.
Main Results:
- The complete genome sequence of UF_RH6 was determined to be 94,715 base pairs.
- UF_RH6 encodes 130 putative proteins.
- Phylogenetic analysis places UF_RH6 within the Caudoviricetes family and Samunavirus genus.
Conclusions:
- The genome sequence of UF_RH6 provides a foundational resource for understanding its biology and potential applications.
- UF_RH6 represents a novel phage candidate for targeting Pseudomonas aeruginosa infections.
- Further studies are warranted to evaluate the efficacy and safety of UF_RH6 in therapeutic contexts.
