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Updated: Jul 28, 2025

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Published on: February 6, 2012

Spermatogonial Transplantation.

Makoto Nagano¹, Xiangfan Zhang²

¹Department of Obstetrics and Gynecology, McGill University and The Research Institute of the McGill University Health Centre, Montreal, QC, Canada. makoto.nagano@mcgill.ca.

Methods in Molecular Biology (Clifton, N.J.)

|May 30, 2023

Summary

Spermatogonial transplantation identifies and quantifies stem cells by assessing their regenerative capacity. This study details the mouse model technique, offering guidance for its application and data interpretation.

Keywords:

Male fertility Microinjection Regeneration Retrospective functional assay Spermatogenesis Stem cell quantification Stem cells

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Area of Science:

Reproductive biology
Stem cell science
Developmental biology

Background:

Spermatogonial stem cells (SSCs) are crucial for male fertility and require functional assays for detection.
Spermatogonial transplantation is the gold standard for identifying and quantifying SSCs based on their regenerative potential.

Purpose of the Study:

To describe the fundamental techniques of spermatogonial transplantation in mice.
To provide practical guidance for performing SSC transplantation and interpreting the resulting data.

Main Methods:

The study details the established protocol for spermatogonial transplantation in a mouse model.
Key steps and considerations for successful transplantation and subsequent analysis are outlined.

Main Results:

Spermatogonial transplantation allows for the functional detection and quantification of SSCs.
The assay endpoint, confirmed by spermatozoa production, takes approximately two months in mice.

Conclusions:

Spermatogonial transplantation is an essential technique for the functional assessment of SSCs.
This guide aims to improve the reproducibility and understanding of SSC transplantation assays.