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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 25, 2026

Planar and Three-Dimensional Printing of Conductive Inks

Planar and Three-Dimensional Printing of Conductive Inks

Published on: December 9, 2011

Ultra-resolution scalable microprinting.

Callum Vidler¹, Kenneth Crozier^2,3,4, David Collins^1,5

¹Department of Biomedical Engineering, University of Melbourne, Melbourne, VIC Australia.

Microsystems & Nanoengineering

|May 30, 2023

Summary

This summary is machine-generated.

This study introduces a low-cost Projection micro stereolithography (PµSL) system achieving 1µm resolution for centimeter-scale microparts. This breakthrough overcomes resolution-volume tradeoffs, enabling advanced 3D materials and bioinspired constructs.

Keywords:

Engineering Optical materials and structures

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Last Updated: Jun 25, 2026

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Published on: December 9, 2011

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Area of Science:

Additive Manufacturing
Microfabrication

Background:

Projection micro stereolithography (PµSL) typically faces a resolution-volume tradeoff, limiting micropart production.
High-resolution, large-volume microstructures are crucial for hierarchical materials, microfluidics, and bioinspired designs.

Discussion:

Investigated PµSL scalability factors: energy dosage, resin composition, cure depth, and feature resolution.
Developed a novel exposure composition technique to significantly enhance printed feature resolution.
Achieved 1µm optical resolution in a centimeter-scale PµSL system, surpassing previous limitations.

Key Insights:

Demonstrated a low-cost PµSL system capable of producing micro-structured parts with unprecedented detail and scale.
Successfully overcame the inherent resolution-volume limitations in digital light processing-based microfabrication.
Validated a new exposure strategy for improving microfabrication resolution.

Outlook:

This advancement is poised to accelerate progress in 3D metamaterials, tissue engineering, and bioinspired constructs.
The developed system offers a scalable platform for creating complex, high-resolution microstructures.
Potential for broader applications in fields requiring precise microscale fabrication.