High-Resolution Volumetric Imaging and Classification of Organisms with Standard Optical Microscopy

Three-dimensional (3D) characterization of organisms is important for the study of cellular phenotypes, structural organization, and mechanotransduction. Existing optical techniques for 3D imaging rely on focus stacking or complex multiangle projection. Focus stacking has deleterious axial resolution due to the one-angle optical projection. Herein, we achieve high-resolution 3D imaging and classification of organisms based on standard optical microscopy coupled to optothermal rotation. Through a seamless fusion of optical trapping and rotation of organisms on a single platform, our technique is applicable to any organism suspended in clinical samples, enabling contact-free and biocompatible 3D imaging. Moreover, when applying deep learning to distinguish different types of biological cells with high similarity, we demonstrate that our platform improves the classification accuracy (96% vs 85%) while using one-tenth the number of training samples compared with conventional deep-learning-based classification.