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Microbiota transfer following liver surgery involves microbial extracellular vesicle migration that affects liver
Shmuel J Cohen1,2, Guy Meyerovich1,2, Simcha Blank1
1Laboratory of the Immuno-HPB and Transplant Surgery, Department of HPB and Transplant Surgery, Tel-Aviv Sourasky Medical Center Affiliated to the Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
Hepatology Communications
|May 31, 2023
Summary
Probiotic-derived vesicles rapidly reach the liver after surgery, enhancing immune cell clearance and improving liver regeneration. These findings offer new insights into the gut-liver axis and post-surgical recovery.
Area of Science:
- Microbiology
- Immunology
- Hepatology
Background:
- Perioperative probiotic use may reduce complications and aid liver recovery after surgery.
- Mechanisms of probiotic influence on the gut microbiome during the perioperative period are unclear.
- This study investigates the direct effects of probiotic microbiota-derived vesicles on liver cells.
Purpose of the Study:
- To elucidate the direct biological effects of probiotic-derived vesicles on host liver cells.
- To understand the role of these vesicles in liver recovery during the perioperative period.
- To explore the impact on immune cell interactions and adhesion molecule expression in the liver.
Main Methods:
- Probiotic-derived vesicles (pbMVs) were isolated from Lactobacillus, Bifidobacterium, and Lactococcus species.
- Mice underwent sham laparotomy or partial hepatectomy, followed by pbMV administration.
- In vivo tracking, flow cytometry, and qRT-PCR were used to analyze intrahepatic cellular and molecular changes.
Main Results:
- Administered pbMVs rapidly translocated to the liver post-surgery.
- pbMVs enhanced neutrophil clearance and modulated inflammatory markers (IL6, IL10, IL17).
- pbMVs reduced intrahepatic adhesion molecules (VCAM1, ICAM2) and improved liver regeneration.
Conclusions:
- Probiotic vesicles provide mechanistic insights into the gut-liver axis post-surgery.
- pbMVs reduce adhesion molecule expression and modulate immune cell invasion.
- This modulation leads to improved liver immunity and enhanced recovery after hepatic surgery.
