Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Calorie restriction modulates beta cell IP<sub>3</sub>R activity to regulate Ca<sup>2+</sup> homeostasis and cell network connectivity.

Cell calcium·2026
Same author

Presenilin-1 controls glycolysis and identity of pancreatic beta cells.

Communications biology·2026
Same author

Pancreatic α-cells are functionally heterogeneous and sex-dependently regulated by neighboring endocrine cells.

Communications biology·2026
Same author

Effects of Arginine Vasopressin on Islet Cells in Pancreatic Tissue Slices: Glucose-Dependent Modulation of Alpha and Beta Cell Activity.

bioRxiv : the preprint server for biology·2026
Same author

[Antihyperglycemic treatment of type 2 diabetes mellitus (Update 2026)].

Wiener klinische Wochenschrift·2026
Same author

Effects of Intermittent Fasting-Mimicking Diet on Pancreatic Islet Plasticity: Immunohistochemical, Ultrastructural, and Metabolic Profiles.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Related Experiment Video

Updated: Jul 28, 2025

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells
08:03

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

Published on: November 26, 2019

8.3K

Tracking Ca2+ Dynamics in NOD Mouse Islets During Spontaneous Diabetes Development.

Sandra Postić1, Johannes Pfabe1, Srdjan Sarikas1

  • 1Center for Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria.

Diabetes
|May 31, 2023
PubMed
Summary

Functional changes in alpha and beta cells during type 1 diabetes (T1D) development were studied in NOD mice. Researchers observed potential beta-cell recovery and alpha-cell synchronization, offering insights into T1D progression.

More Related Videos

Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices
10:49

Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

Published on: April 13, 2021

4.1K
Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
10:09

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

Published on: May 11, 2015

9.5K

Related Experiment Videos

Last Updated: Jul 28, 2025

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells
08:03

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

Published on: November 26, 2019

8.3K
Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices
10:49

Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

Published on: April 13, 2021

4.1K
Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
10:09

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

Published on: May 11, 2015

9.5K

Area of Science:

  • Endocrinology
  • Immunology
  • Cell Biology

Background:

  • Type 1 diabetes (T1D) involves autoimmune destruction of pancreatic beta cells, leading to insulin deficiency.
  • The functional dynamics of alpha and beta cells during T1D development remain poorly understood.
  • Nonobese diabetic (NOD) mice serve as a valuable model for studying spontaneous T1D development.

Purpose of the Study:

  • To investigate the functional changes in pancreatic alpha and beta cells during the progression of T1D in NOD mice.
  • To assess the impact of T1D development on cytosolic calcium ion ([Ca2+]c) dynamics in islet cells.
  • To identify potential windows of functional recovery in beta cells and functional synchronization in alpha cells.

Main Methods:

  • Utilized high spatiotemporal resolution imaging of cytosolic calcium ([Ca2+]c) dynamics.
  • Analyzed [Ca2+]c dynamics in fresh pancreas tissue slices from female NOD mice.
  • Tracked dynamic changes in alpha and beta cells throughout T1D development, from islet inflammation to destruction phases.

Main Results:

  • In beta cells, 8 mmol/L glucose-induced synchronized short [Ca2+]c events diminished during islet inflammation, while long [Ca2+]c events became more sensitive to lower glucose levels.
  • During islet destruction, short [Ca2+]c events in a subset of beta cells resumed at high glucose, and long [Ca2+]c events increased at substimulatory glucose.
  • Alpha cell glucose sensitivity of [Ca2+]c events persisted, with occasional synchronicity observed in late-stage destruction.

Conclusions:

  • Beta cell function exhibits dynamic changes and potential recovery windows during T1D development.
  • Alpha cells maintain glucose sensitivity and may exhibit functional synchronization in advanced T1D.
  • These findings provide novel insights into islet cell function during the pathogenesis of type 1 diabetes.