Related Experiment Video

Updated: Jul 28, 2025

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity
07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

7.5K

PSMD13 inhibits NF-κB pathway by targeting TAK1 for K63-linked ubiquitination in miiuy croaker (Miichthys miiuy)

Qi Li1, Ya Chen1, Pengfei Wang1

  • 1Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.

Fish & Shellfish Immunology
|May 31, 2023
PubMed

Insights

Proteasome 26S subunit, non-ATPase (PSMD) 13 regulates innate immunity by inhibiting the NF-κB pathway. PSMD13 targets transforming growth factor-β activated kinase 1 (TAK1), reducing its ubiquitination and expression.

Area of Science:

  • Innate immunity
  • Molecular biology
  • Cellular signaling

Background:

  • Transforming growth factor-β activated kinase 1 (TAK1) is crucial in Toll-like receptor (TLR)-mediated NF-κB signaling.
  • The role of Proteasome 26S subunit, non-ATPase (PSMD) 13 in innate immune regulation remains largely unknown.

Purpose of the Study:

  • To elucidate the mechanism by which PSMD13 regulates innate immunity.
  • To investigate the interaction between PSMD13 and the NF-κB pathway.

Main Methods:

  • Analyzing PSMD13 mRNA expression levels following Vibrio harveyi stimulation.
  • Investigating the effect of PSMD13 on TAK1 ubiquitination and NF-κB pathway activity.

Main Results:

  • PSMD13 mRNA expression significantly increased upon Vibrio harveyi challenge.
  • PSMD13 was found to inhibit the NF-κB pathway by targeting TAK1.
  • PSMD13 significantly reduced K63-linked ubiquitination of TAK1, thereby inhibiting its expression.

Conclusions:

  • PSMD13 plays a regulatory role in innate immunity by inhibiting the TAK1-mediated NF-κB pathway.
  • This study expands understanding of PSMD family functions in immune responses.
  • Provides novel insights into mammalian innate immune regulation mechanisms.
Keywords:
Innate immunePSMD13TAK1Ubiquitination

More Related Videos

Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis14:58

Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis

Published on: February 13, 2014

14.4K
Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway07:15

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

489

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.5K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.7K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.5K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.0K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.5K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.1K