Seminal prolactin is associated with HSP90 transcript content in ejaculated spermatozoa

Area of Science:

• Reproductive Biology
• Andrology
• Biochemistry

Background:

• Oxidative stress imbalance is implicated in male infertility.
• Seminal prolactin (PRL) and heat shock protein 90 (HSP90) are potential factors in male reproductive health.

Purpose of the Study:

• To investigate the association between seminal PRL levels and sperm parameters.
• To evaluate the relationship between seminal PRL and HSP90 transcript abundance in spermatozoa.
• To assess the diagnostic potential of seminal PRL in male infertility.

Main Methods:

• Seminal PRL levels were measured in normozoospermic donors and infertile men.
• HSP90 transcript abundance in ejaculated spermatozoa was quantified using real-time PCR.
• Semen parameters including sperm count and progressive motility were assessed.

Main Results:

• Infertile men exhibited significantly lower seminal PRL concentrations compared to fertile controls.
• Seminal PRL levels positively correlated with sperm count and progressive motility.
• Spermatozoa from infertile men showed higher HSP90 transcript abundance, which negatively correlated with progressive motility.
• Higher seminal PRL levels were associated with lower sperm HSP90 transcript abundance.

Conclusions:

• Seminal PRL levels are associated with semen quality and HSP90 transcript abundance.
• Seminal PRL may play a role in maintaining seminal antioxidant capacity, contributing to male fertility.
• Seminal PRL demonstrates potential as a diagnostic and prognostic biomarker for male infertility.