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Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation
Dilem Shakir1, Michael Batie1, Sonia Rocha2
1Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.
Methods in Molecular Biology (Clifton, N.J.)
|May 31, 2023
Abstract:
Hypoxia and its signalling pathway play a key role in human physiology and a variety of diseases. Alterations in histone methylation coordinate transcriptional responses to hypoxia. Here, we detail a fixed cell immunofluorescence method for quantifying hypoxia-induced changes in histone methylation, exemplified by the measurement of H3K27me3.
