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Updated: Jul 28, 2025
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Published on: April 1, 2017
Affinity-Purification Combined with Crosslinking Mass Spectrometry for Identification and Structural Modeling of
1Department of Clinical Sciences Lund, Division of Infection Medicine, Lund University, Lund, Sweden. lotta.happonen@med.lu.se.
Abstract:
Host-pathogen protein-protein interactions are highly complex and dynamic and mediate key steps in pathogen adhesion to host, host invasion, and colonization as well as immune evasion. In bacteria, these interactions most often involve specialized virulence factors or effector proteins that specifically target central host proteins. Here, I present a mass spectrometry-based proteomics approach starting with the identification of host-pathogen interactions by affinity-purification followed by mapping the specific host-pathogen protein-protein interaction interfaces by crosslinking mass spectrometry and structural modeling of the complexes.
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