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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 28, 2025

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

Published on: November 27, 2014

Quantification of Phagocytosis Using Flow Cytometry.

Therese de Neergaard¹, Pontus Nordenfelt²

¹Department of Clinical Sciences Lund, Faculty of Medicine, Division of Infection Medicine, Lund University, Lund, Sweden.

Methods in Molecular Biology (Clifton, N.J.)

|May 31, 2023

Summary

This summary is machine-generated.

Quantifying phagocytosis accurately is crucial for research. This study presents a robust flow cytometry method using dose-response principles to improve sensitivity and reproducibility in phagocytosis assays.

Keywords:

Bacteria Flow cytometry Opsonization Phagocytosis Streptococcus pyogenes

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Last Updated: Jul 28, 2025

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Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

Published on: December 7, 2019

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Phagocytosis is a critical cellular process with broad research applications.
Accurate quantification of phagocytosis is often challenging due to experimental design and analysis complexities.
Standard methods may overlook critical factors like reaction volume, time, and concentration ratios.

Purpose of the Study:

To develop a robust, high-throughput, and user-friendly method for quantifying phagocytosis using flow cytometry.
To enhance the sensitivity and reproducibility of phagocytosis assays through optimized experimental design and analysis.
To provide a standardized approach for comparable phagocytosis measurements across different experiments.

Main Methods:

Fluorescent double staining of prey particles followed by optional opsonization.
Incubation of labeled prey with phagocytes across a range of ratios.
Flow cytometry acquisition for population and single-cell analysis, distinguishing adhesion from internalization.
Application of dose-response curve principles for experimental design and data analysis.

Main Results:

The developed flow cytometry approach offers high sensitivity and robustness for phagocytosis quantification.
The method allows for the differentiation between phagocyte adhesion and internalization.
Demonstrated successful application using an example protocol for Streptococcus pyogenes and THP-1 cells.

Conclusions:

Implementing dose-response principles in experimental design and analysis significantly improves phagocytosis quantification.
This flow cytometry-based method provides a reproducible and sensitive means to measure phagocytosis.
The approach is adaptable to various phagocytosis assays, enhancing comparability and reliability of results.