Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

4.3K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.3K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

954
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
954
Combinatorial Gene Control02:33

Combinatorial Gene Control

8.4K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.4K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.1K
3.1K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K
Dosage Compensation02:50

Dosage Compensation

6.2K
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with  distinct numbers of X chromosomes will...
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The research progress in determining lignocellulosic content by near infrared reflectance spectroscopy technology].

Guang pu xue yu guang pu fen xi = Guang pu·2014
Same author

Human papillomavirus prevalence is high in oral samples of patients with tonsillar and base of tongue cancer.

Oral oncology·2014
Same author

Neuroprotective effects of oxysophocarpine on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion.

Pharmaceutical biology·2014
Same author

Intertriginous cutaneous mastocytosis in a 16-year-old boy.

International journal of dermatology·2014
Same author

Lycium barbarum polysaccharide prevents focal cerebral ischemic injury by inhibiting neuronal apoptosis in mice.

PloS one·2014
Same author

Clinical analysis on 33 patients with hypothalamic syndrome in Chinese children.

Journal of pediatric endocrinology & metabolism : JPEM·2014

Related Experiment Video

Updated: Jul 28, 2025

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.5K

Stuxnet fine-tunes Notch dose during development using a functional Polycomb response element.

Tao He1,2, Yu Fan1, Juan Du3

  • 1Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University, Beijing 100871, China.

Development (Cambridge, England)
|June 1, 2023
PubMed
Summary
This summary is machine-generated.

Epigenetic regulator Stuxnet (Stx) controls Notch signaling by opposing Polycomb repressive complex 1 (PRC1). This study identifies a direct epigenetic mechanism regulating Notch receptor levels during development.

Keywords:
NotchEpigenetic regulationPRC1PREStuxnet

More Related Videos

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

8.5K
An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

6.7K

Related Experiment Videos

Last Updated: Jul 28, 2025

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.5K
Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

8.5K
An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

6.7K

Area of Science:

  • Developmental biology
  • Epigenetics
  • Molecular signaling

Background:

  • Notch signaling is crucial for development and sensitive to receptor levels.
  • Epigenetic regulation influences cellular responses to stress.
  • The direct epigenetic control of Notch receptor dose remains largely unknown.

Purpose of the Study:

  • To investigate the role of the epigenetic regulator Stuxnet (Stx) in Drosophila development.
  • To determine if Notch receptor levels are epigenetically regulated.
  • To identify the epigenetic mechanisms controlling Notch signaling dosage.

Main Methods:

  • Studied Stuxnet's role in Drosophila developmental signaling.
  • Utilized DamID-seq and ChIP-seq to identify Polycomb group (PcG) binding sites.
  • Performed in situ deletion of a validated Polycomb response element (PRE).

Main Results:

  • Stuxnet promotes Notch receptor mRNA expression by counteracting Polycomb repressive complex 1 (PRC1).
  • Notch is a direct PRC1 target, with a validated Polycomb response element (PRE).
  • Deletion of the PRE increases Notch expression and causes phenotypes of Notch hyperactivation.

Conclusions:

  • Epigenetic regulation by PRC1 directly fine-tunes Notch activity dose.
  • Stuxnet antagonizes PRC1 to modulate Notch receptor expression.
  • The findings highlight the physiological relevance of omics-based PcG binding in development.