Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents.

Area of Science:

• Biotechnology
• Biochemical Engineering
• Materials Science

Background:

• Adeno-associated viruses (AAVs) are crucial for gene therapy, but their purification faces challenges with conventional chromatography.
• Packed bed chromatography exhibits diffusion and pressure drop limitations, impacting process efficiency.

Purpose of the Study:

• To investigate the efficacy of electrospun cellulose-based nanofiber adsorbents for adeno-associated virus serotype 5 (AAV5) capture.
• To evaluate dynamic binding capacity, pressure drop, and AAV5 recovery at low residence times.

Main Methods:

• Utilized affinity cellulose-based nanofiber adsorbents for AAV5 capture.
• Assessed dynamic binding capacity, pressure drop, and AAV5 recovery at residence times under 5 seconds.

Main Results:

• Achieved a dynamic binding capacity exceeding 1.0 × 10^14 TP mL^-1 at a 1.6-second residence time.
• Observed a low pressure drop (0.14 MPa) after processing over 2,000 column volumes.
• Attained 80% AAV5 recovery with impurity removal comparable to traditional methods.

Conclusions:

• Cellulose-based nanofiber adsorbents demonstrate high binding capacity and efficient AAV5 capture at rapid flow rates.
• These novel adsorbents can potentially streamline gene therapy manufacturing by reducing unit operations, process time, and costs.