Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.1K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.1K
Biological Clocks and Seasonal Responses02:45

Biological Clocks and Seasonal Responses

34.8K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
34.8K
Mechanism of Ciliary Motion01:05

Mechanism of Ciliary Motion

3.7K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
3.7K
Microtubules in Signaling01:22

Microtubules in Signaling

1.7K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
1.7K
Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response01:15

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

94
Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...
94
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.8K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thioamide Compound H0802 Enhances Hypoxia Tolerance by Mimicking Hypoxia-Adaptive Reprogramming of Glucose and Oxygen Metabolism.

Antioxidants (Basel, Switzerland)·2026
Same author

Tannic acid inhibits viral replication by disrupting nucleocapsid condensation.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Prasugrel inhibits TLR7-driven autoimmunity in systemic lupus erythematosus by acetylating cGAS.

Nature communications·2026
Same author

The glycolytic metabolite phosphoenolpyruvate restricts cGAS-driven inflammation to promote healthy aging.

Nature aging·2026
Same author

Disruption of the nucleocapsid-RNA condensation by punicalagin is a broad-spectrum antiviral approach.

Fundamental research·2026
Same author

Identification of KHS-101 as a Transcription Factor EB Activator to Promote α-Synuclein Degradation.

International journal of molecular sciences·2026

Related Experiment Video

Updated: Jul 28, 2025

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
05:46

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

Published on: January 24, 2013

21.4K

Rhythmic cilia changes support SCN neuron coherence in circadian clock.

Hai-Qing Tu1, Sen Li1, Yu-Ling Xu1

  • 1Nanhu Laboratory, National Center of Biomedical Analysis, Beijing, China.

Science (New York, N.Y.)
|June 1, 2023
PubMed
Summary
This summary is machine-generated.

Primary cilia in the suprachiasmatic nucleus (SCN) are essential for maintaining the internal clock

More Related Videos

Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus
10:06

Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus

Published on: February 15, 2011

35.4K
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
06:53

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

8.4K

Related Experiment Videos

Last Updated: Jul 28, 2025

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
05:46

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

Published on: January 24, 2013

21.4K
Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus
10:06

Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus

Published on: February 15, 2011

35.4K
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
06:53

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

8.4K

Area of Science:

  • Neuroscience
  • Chronobiology
  • Cell Biology

Background:

  • The suprachiasmatic nucleus (SCN) is the master circadian clock in mammals, coordinating physiological rhythms.
  • Intercellular coupling among SCN neurons is crucial for maintaining circadian clock coherence and robustness.
  • Environmental perturbations can disrupt circadian rhythms, highlighting the need for mechanisms that ensure clock stability.

Purpose of the Study:

  • To investigate the role of primary cilia in intercellular coupling within the SCN.
  • To determine if primary cilia in specific SCN neurons contribute to the robustness of the internal circadian clock.
  • To elucidate the molecular mechanisms, including Sonic Hedgehog (Shh) signaling, by which cilia influence SCN function.

Main Methods:

  • Utilized genetic ablation of ciliogenesis in neuromedin S-producing (NMS) neurons in mice.
  • Assessed the impact of cilia deficiency on circadian clock phase shifting under simulated jet-lag conditions.
  • Analyzed circadian rhythms of individual SCN neurons in cilia-deficient slices and investigated Sonic Hedgehog (Shh) signaling pathways.

Main Results:

  • Primary cilia in NMS neurons exhibit circadian rhythmicity in abundance and length.
  • Genetic ablation of cilia in NMS neurons led to rapid internal clock phase shifts under jet-lag conditions.
  • SCN neurons lacking cilia showed desynchronized rhythms after external perturbations, and Shh signaling inactivation mimicked cilia ablation effects.

Conclusions:

  • Primary cilia are essential for intercellular coupling among SCN neurons, ensuring robust circadian clock function.
  • Rhythmic changes in cilia modulate Shh signaling and clock gene expression, contributing to circadian coherence.
  • Cilia-mediated Shh signaling in the SCN is a critical mechanism for maintaining internal clock stability against environmental disruptions.