Suspended phosphorus sustains algal blooms in a dissolved phosphorus-depleted lake

The expansion of algal bloom in surface waters is a global problem in the freshwater ecosystem. Differential reactivity of organic phosphorus (P_o) compounds from organic debris, suspended particulate matter (SPM), and sediment towards hydrolysis can dictate the extent of supply often limited inorganic P (P_i) for algal growth, thereby controlling the extent of bloom. Here, we combined solution P-31 nuclear magnetic resonance (³¹P NMR), sequential extraction, enzymatic hydrolysis, and 16S rRNA measurements to characterize speciation and biogeochemical cycling of P in Lake Erhai, China. Lower ratios of diester-P/monoester-P in SPM in January (mean 0.09) and July (0.14) than that in April (0.29) reflected the higher degree of diester-P remineralization in cold and warm months. Both H₂O-P_i and P_o were significantly higher in SPM (mean 1580 mg ·kg^-1 and 1618 mg ·kg^-1) than those in sediment (mean 8 mg ·kg^-1 and 387 mg ·kg^-1). In addition, results from enzymatic hydrolysis experiments demonstrated that 61% P_o in SPM and 58% in sediment in the H₂O, NaHCO₃, and NaOH extracts could be hydrolyzed. These results suggested that H₂O-P_i and P_o from SPM were the primarily bioavailable P sources for algae. Changes of P_i contents (particularly H₂O-P_i) in algae and alkaline phosphatase activity (APA) during the observation periods were likely to be controlled by the strategies of P uptake and utilization of algae. P remobilization/remineralization from SPM likely resulted from algae and bacteria (e.g., Pseudomonas). Collectively, these results provide important insights that SPM P could sustain the algal blooms even if the dissolved P was depleted in the water column.