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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 28, 2025

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Advanced flow cytometry for biomedical applications.

Kai Pang¹, Sihan Dong², Yuxi Zhu¹

¹School of Instrument Science and Opto-Electronics Engineering of Beijing Information Science & Technology University, Beijing, China.

Journal of Biophotonics

|June 1, 2023

Summary

This summary is machine-generated.

Advanced flow cytometry (FC) offers new capabilities for cell analysis, expanding applications in cancer detection and microbiology. This review highlights recent advancements and trends to accelerate clinical translation of these powerful techniques.

Keywords:

Raman flow cytometry flow cytometry imaging flow cytometry in vivo flow cytometry microfluidic flow cytometry photoacoustic flow cytometry

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Area of Science:

Biotechnology and Biomedical Engineering
Cellular and Molecular Biology

Background:

Flow cytometry (FC) is a powerful technique for analyzing cell populations.
Recent decades have seen significant advancements in FC technologies.
These innovations have expanded FC's utility in research and clinical settings.

Purpose of the Study:

To provide a comprehensive overview of advanced flow cytometry techniques.
To elucidate the mechanisms and practical applications of novel FC methods.
To identify emerging trends and promote awareness of advanced FC.

Main Methods:

Review of recent literature on advanced flow cytometry.
Analysis of new functionalities in techniques like in vivo photoacoustic FC, coherent Raman FC, and microfluidic FC.
Discussion of applications in cancer detection, microbiology, and nucleic acid analysis.

Main Results:

Advanced FC techniques offer enhanced capabilities for detecting and measuring cellular characteristics.
New FC methods broaden research tools for critical areas like oncology and infectious diseases.
The review details mechanisms and practical insights into cutting-edge FC applications.

Conclusions:

Advanced flow cytometry represents a significant leap in cell analysis technology.
Increased awareness and understanding of these techniques can accelerate their adoption in clinical practice.
Future developments promise further expansion of FC applications in diagnostics and therapeutics.