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Related Experiment Video

Updated: Jul 28, 2025

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
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PHDs-seq: a large-scale phenotypic screening method for drug discovery through parallel multi-readout quantification.

Jun Li1,2, Jun Chi3, Yang Yang1,3

  • 1State Key Laboratory of Natural and Biomimetic Drugs, MOE Key Laboratory of Cell Proliferation and Differentiation, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, College of Future Technology, Peking University, Beijing, 100871, China.

Cell Regeneration (London, England)
|June 1, 2023
PubMed
Summary
This summary is machine-generated.

A new PHDs-seq (Probe Hybridization based Drug screening by sequencing) platform enables high-throughput screening of compounds for stem cell research. This method identified ABT869 as a compound that can reprogram keloid fibroblasts into adipocytes.

Keywords:
ABT869Adipocyte reprogrammingHigh-throughput screeningPHDs-seq

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Area of Science:

  • Biotechnology
  • Stem Cell Biology
  • Drug Discovery

Background:

  • High-throughput phenotypic screening is crucial for drug development and stem cell research.
  • Conventional methods struggle to simultaneously detect multiple cell fate biomarkers and assess cell transition.
  • Accurate assessment of cell fate determination and transition requires advanced screening techniques.

Purpose of the Study:

  • To introduce the PHDs-seq (Probe Hybridization based Drug screening by sequencing) platform for evaluating compound effects on transcriptional biomarkers.
  • To demonstrate the utility of PHDs-seq in profiling cell fate determination markers during adipocyte reprogramming.
  • To screen a panel of compounds for their ability to induce fibroblast-to-adipocyte reprogramming.

Main Methods:

  • Development and validation of the PHDs-seq platform for accuracy, sensitivity, and reproducibility.
  • Profiling of cell fate determination markers in adipocyte reprogramming from dermal fibroblasts.
  • Screening of 128 signalling-related compounds using PHDs-seq to identify reprogramming agents.

Main Results:

  • PHDs-seq demonstrated high accuracy, sensitivity, and reproducibility in molecular and cellular assays.
  • The platform successfully profiled multiple biomarkers related to cell fate determination.
  • ABT869, a VEGFR/PDGFR inhibitor, was identified as a compound that promotes fibroblast-to-adipocyte transition.

Conclusions:

  • The PHDs-seq platform offers a powerful and accurate method for high-throughput drug screening in stem cell research.
  • This technology can be applied to explore molecular mechanisms underlying disease development and cell reprogramming.
  • PHDs-seq facilitates the discovery of novel compounds for therapeutic applications in regenerative medicine.