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Updated: Jul 28, 2025
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Histological-Based Stainings Using Free-Floating Tissue Sections
Published on: August 25, 2020
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What is happening to immunohistochemistry?
1Internal Medicine and Center for Hypothalamic Research, UT Southwestern Medical Center, Dallas, Texas, USA.
Summary
Immunohistochemistry use is declining due to unreliable antibodies and new RNA in situ hybridization methods. This highlights issues in laboratory and publishing practices affecting research reproducibility.
Area of Science:
- Biomedical research
- Laboratory techniques
- Scientific publishing
Background:
- Immunohistochemistry is a widely adopted laboratory method.
- Recent years show a notable decrease in PubMed entries for immunohistochemistry.
- This trend is linked to concerns about research antibody reliability.
Purpose of the Study:
- To explore factors influencing laboratory and publishing practices.
- To examine the decline in immunohistochemistry usage.
- To discuss the impact of unreliable reagents and alternative techniques.
Main Methods:
- Literature review of PubMed entries.
- Analysis of trends in scientific publications.
- Discussion of laboratory best practices and publishing standards.
Main Results:
- A significant decrease in immunohistochemistry publications was observed.
- Increased awareness of antibody validation issues is a contributing factor.
- Emergence of RNA in situ hybridization offers an alternative.
Conclusions:
- The decline in immunohistochemistry reflects broader challenges in research reproducibility.
- Good laboratory and publishing practices are crucial for reliable scientific outcomes.
- Adoption of validated reagents and advanced techniques is essential for future research.
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