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m6A‑mediated LINC02038 inhibits colorectal cancer progression via regulation of the FAM172A/PI3K/AKT pathway via

Wenjun Liu1, Zilang Zhang2, Xitu Luo1

  • 1The First Department of General Surgery, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. China.

International Journal of Oncology
|June 2, 2023
PubMed

Insights

Long noncoding RNA LINC02038 acts as a tumor suppressor in colorectal cancer (CRC) by regulating miR-552-5p and FAM172A. Its m6A modification influences CRC progression and metastasis, suggesting it as a potential biomarker.

Area of Science:

  • Molecular Biology
  • Oncology
  • Epigenetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in various cancers, but their specific roles in colorectal cancer (CRC) require further elucidation.
  • Understanding the molecular mechanisms of lncRNAs in CRC is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the molecular mechanism of LINC02038 in the progression of colorectal cancer (CRC).
  • To explore the potential of LINC02038 as a biomarker and therapeutic target for CRC.

Main Methods:

  • Quantitative analysis of LINC02038 expression in CRC tissues and adjacent tissues.
  • Cell proliferation, migration, invasion, and apoptosis assays following LINC02038 overexpression.
  • Bioinformatics analysis, RNA immunoprecipitation, luciferase reporter assays, and methylated RNA immunoprecipitation (MeRIP)-qPCR to elucidate molecular interactions.
  • Correlation analysis between LINC02038 expression and clinicopathological features in CRC patients.

Main Results:

  • LINC02038 expression was significantly decreased in CRC tissues.
  • Overexpression of LINC02038 suppressed CRC cell proliferation, migration, and invasion while promoting apoptosis.
  • LINC02038 acted as a molecular sponge for miR-552-5p, inhibiting the degradation of its target gene, FAM172A.
  • METTL3-mediated m6A modification of LINC02038, identified by YTHDF2, promoted CRC progression via the PI3K/AKT pathway.
  • Reduced LINC02038 expression correlated with increased lymphatic and distant metastasis in CRC patients.

Conclusions:

  • The m6A/LINC02038/miR-552-5p/FAM172A axis represents a novel anti-tumor pathway in colorectal cancer.
  • LINC02038 functions as a tumor suppressor and holds potential as a diagnostic biomarker and therapeutic target for CRC.
Keywords:
LINC02038N6‑methyladenosineceRNAcolorectal cancerprogression

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