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Related Experiment Video

Updated: Jul 28, 2025

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
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Delivery platforms for broadly neutralizing antibodies.

Lok R Joshi1, Nicolás M S Gálvez1, Sukanya Ghosh2

  • 1Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard University, Cambridge, Massachusetts.

Current Opinion in HIV and AIDS
|June 2, 2023
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Summary
This summary is machine-generated.

Gene-based delivery of broadly neutralizing antibodies (bNAbs) offers a promising alternative to passive antibody administration for HIV prevention and treatment. Advances in DNA, mRNA, and viral vector technologies are overcoming limitations for effective HIV interventions.

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Area of Science:

  • Immunology and Virology
  • Gene Therapy and Biotechnology

Background:

  • Passive administration of broadly neutralizing antibodies (bNAbs) is a strategy for HIV prevention and treatment.
  • Current challenges with passive transfer include the need for recurrent administrations.
  • Gene-based delivery approaches are being developed to overcome these limitations.

Approach:

  • DNA-encoded monoclonal antibodies (DMAbs) show promise in preclinical studies and are being tested in human vaccine trials.
  • mRNA-based delivery of bNAbs has advanced significantly, accelerated by COVID-19 vaccine development.
  • Viral vectors, particularly adeno-associated virus (AAV), are effective for bNAb delivery in preclinical and early human studies.
  • Genome editing is being explored to engineer B cells for sustained antibody production.

Key Points:

  • Various gene-based platforms (DNA, mRNA, viral vectors) are being successfully applied for bNAb delivery in HIV models.
  • Engineered B cells represent a novel approach for continuous antibody generation.
  • Each gene-delivery platform has distinct advantages and disadvantages.

Conclusions:

  • Gene-based delivery of bNAbs holds significant potential for HIV treatment and prevention.
  • Further advancements in gene delivery efficiency and reduced immunogenicity are crucial for widespread implementation.
  • Overcoming technical challenges in gene-based bNAb delivery offers a clear path toward practical HIV interventions.