Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A case report of jejunogastric intussusception complicated by torsion: 17 years after subtotal gastrectomy.

Frontiers in surgery·2026
Same author

KPNA2 knockdown suppresses gastric cancer progression through modulation of the β-catenin/EMT signaling axis, inducing cell cycle arrest, apoptosis, and reduced metastatic capacity.

Journal of molecular histology·2026
Same author

Identification of Antibacterial Hits Associated with Penicillin-Binding Protein 2 in <i>Escherichia coli</i> Using a Comprehensive Property Spectrum and Fivefold Maximum Drug-Likeness Strategy.

Drug design, development and therapy·2026
Same author

Network Pharmacology Reveals the Therapeutic Potential of BBB-Permeable Compounds from <i>Lonicera caerulea</i> for Alzheimer's Disease and Lipid Metabolism Disorders.

International journal of molecular sciences·2026
Same author

A Fivefold Maximum Drug-Likeness Strategy for Prioritizing Antibacterial Candidates Against <i>Escherichia coli</i>.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Predicting Aneurysm Occlusion After Pipeline Embolization: an Ensemble Model Using Angiographic Parametric Imaging.

AJNR. American journal of neuroradiology·2026

Related Experiment Video

Updated: Jul 28, 2025

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

21.2K

Portable and integrated microfluidic flow control system using off-the-shelf components towards organs-on-chip

Haoyu Zhu1, Gürhan Özkayar1, Joost Lötters1,2,3

  • 1Department of Precision and Microsystems Engineering, Delft University of Technology, Mekelweg 2, Zuid-Holland, 2628CD, Delft, The Netherlands.

Biomedical Microdevices
|June 2, 2023
PubMed
Summary

Researchers developed a compact, integrated fluidic system for organ-on-a-chip (OoC) experiments using off-the-shelf parts and 3D printing. This portable system enables precise media control for lung-on-a-chip applications, overcoming previous limitations in portability and integration.

Keywords:
Flow control schemeFluid flow controlFluid handling systemOrgan-on-a-chipPortabilitySystem designSystem integration

More Related Videos

Generation of a Human iPSC-Based Blood-Brain Barrier Chip
10:20

Generation of a Human iPSC-Based Blood-Brain Barrier Chip

Published on: March 2, 2020

12.7K
Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
14:48

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

4.1K

Related Experiment Videos

Last Updated: Jul 28, 2025

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

21.2K
Generation of a Human iPSC-Based Blood-Brain Barrier Chip
10:20

Generation of a Human iPSC-Based Blood-Brain Barrier Chip

Published on: March 2, 2020

12.7K
Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
14:48

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

4.1K

Area of Science:

  • Biotechnology
  • Microfluidics
  • Bioengineering

Background:

  • Organ-on-a-chip (OoC) devices require precise media control, but current fluidic systems are bulky and lack integration, hindering portability.
  • Existing fluidic setups often involve numerous external components and tubing, limiting their application in diverse experimental settings.

Purpose of the Study:

  • To explore the integration limits of fluidic systems for OoC devices using readily available components.
  • To develop a compact, portable, and integrated fluidic control platform for OoC applications, specifically demonstrated for lung-on-a-chip experiments.

Main Methods:

  • Utilized off-the-shelf fluidic control components, including a vacuum pump, switch valve, and flow/pressure controllers.
  • Employed 3D printing to fabricate a custom platform box for component integration, enabling flexible arrangement and system customization.
  • Designed a flow control configuration leveraging vacuum to achieve fluctuation-free flow and minimize component count.

Main Results:

  • Successfully constructed a demonstrator system for lung-on-a-chip experiments, measuring 290x240x37 mm and weighing 4.8 kg.
  • The system supports liquid flow rates from 1.5 to 68 µL/min and applies cyclic vacuum (280 mbar at 0.5 Hz) for mechanical cell stimulation.
  • The battery-operated, modular system is compatible with standard microscopes and incubators, demonstrating enhanced portability and ease of use.

Conclusions:

  • A compact, integrated, and portable fluidic system for OoC experiments can be realized using standard, off-the-shelf components and 3D printing.
  • Further miniaturization of fluidic control components (pumps, valves, flow controllers) is necessary for achieving even smaller OoC systems with high-resolution flow control.