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Updated: Jul 28, 2025
07:13
Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
Published on: February 13, 2021
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Osteogenic microenvironment affects palatal development through glycolysis
Xia Peng1, Jing Chen1, Yijia Wang1
1Laboratory of Orofacial Development, Laboratory of Molecular Signaling and Stem Cells Therapy, Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tiantan Xili No.4, Beijing, 100050, China.
Differentiation; Research in Biological Diversity
|June 2, 2023
Summary
Mouse embryonic palatal mesenchyme (MEPM) cells show distinct biological properties based on developmental stage. The osteogenic microenvironment impacts MEPM cell proliferation, apoptosis, migration, and stemness, influencing their role in palate development and cleft palate research.
Area of Science:
- Developmental Biology
- Cell Biology
- Tissue Engineering
Background:
- Palate development is a complex process involving cell proliferation, differentiation, and migration.
- Disruptions in palate development can lead to cleft palate (CP), a common congenital birth defect.
- Mouse embryonic palatal mesenchyme (MEPM) cells are a key model for studying palatal development and CP, but the influence of their microenvironment is understudied.
Purpose of the Study:
- To investigate differences in MEPM cells from distinct developmental stages (E13.5 and E15.5).
- To determine the effect of an osteogenic microenvironment on MEPM cell biological properties.
- To elucidate the role of MEPM cell stemness and differentiation capacity in palate development.
Main Methods:
- Comparison of MEPM cells isolated at embryonic day 13.5 (E13.5) and embryonic day 15.5 (E15.5).
- Culture of MEPM cells in routine medium versus osteogenic differentiation medium (OIM).
- Assessment of cell proliferation, cell cycle (S phase), apoptosis, migration, stemness, and differentiation potential.
- Analysis of Lactate dehydrogenase A (LDHA) and Cytochrome c (CytC) expression.
Main Results:
- MEPM cells at E13.5 showed higher proliferation and stemness, while E15.5 cells exhibited a greater tendency towards osteogenesis.
- The osteogenic microenvironment (OIM) reduced proliferation, lengthened S phase, increased apoptosis, and decreased migration in MEPM cells.
- E15.5 MEPM cells were more sensitive to OIM than E13.5 cells, with altered LDHA and CytC expression suggesting a role for glycolysis.
- E13.5 MEPM cells demonstrated stronger stemness, potentially serving as precursors to E15.5 MEPM cells.
Conclusions:
- The developmental stage and microenvironment significantly alter MEPM cell biological properties.
- Osteogenic cues influence MEPM cell behavior, impacting proliferation, apoptosis, migration, and stemness.
- Understanding these microenvironmental effects is crucial for accurate interpretation of MEPM cell models in palate development and CP research.
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