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Updated: Jul 28, 2025
Technique for Intranasal Administration of α-Synuclein Aggregates
Published on: November 8, 2024
Single-dose intranasal administration of α-syn PFFs induce lewy neurite-like pathology in olfactory bulbs
Masanori Sawamura1, Junichiro Ohira1, Rie Hikawa1
1Department of Neurology, Kyoto University Hospital, Kyoto, Japan.
Introduction:
Pathological α-synuclein (α-Syn) propagation may cause Parkinson's disease progression. We aimed to verify whether single-dose intranasal administration of α-Syn preformed fibrils (PFFs) induces α-Syn pathology in the olfactory bulb (OB).
Methods:
A single dose of α-Syn PFFs was administered to the left nasal cavity of wild-type mice. The untreated right side served as a control. The α-Syn pathology of the OBs was examined up to 12 months after the injection.
Results:
Lewy neurite-like aggregates were observed in the OB 6 and 12 months after the treatment.
Conclusions:
These findings suggest that pathological α-Syn can propagate from the olfactory mucosa to the OB and reveal the potential dangers of α-Syn PFFs inhalation.
