A turn-off fluorimetric -aptasensor for early detection of apoptosis inside the cells
Hamed Salmani-Zarchi1, Yasaman-Sadat Borghei2, Maryam Nikkhah3
1Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box: 14115-175, Tehran, Iran.
Abstract:
To detect cytochrome c (Cyt c) as an important biomarker of apoptosis inside the cells, a simple, label-free, fluorometric detection method has been presented. For this purpose, an aptamer/gold nanocluster probe (Aptamer@AuNCs) was produced which could specifically bind to Cyt c leading to fluorescence quenching of AuNCs. The developed aptasensor showed two linear ranges of 1-80 μM and 100-1000 μM and a detection limit of 0.77 μM and 297.5 μM, respectively. This platform was successfully used to assay Cyt c release inside the apoptotic cells and their cell lysate. Aptamer@AuNC due to its enzyme-like properties could replace antibodies in Cyt c detection by conventional blotting techniques.
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