Highly sensitive cholesterol concentration trace detection based on a microfiber optic-biosensor enhanced specificity...

Area of Science:

• Biomedical Engineering
• Chemical Sensing
• Optoelectronics

Background:

• Cholesterol detection is crucial for diagnosing cardiovascular diseases.
• Existing biosensors often face limitations in sensitivity and selectivity.
• Optic-fiber biosensors offer advantages for real-time and in-situ measurements.

Purpose of the Study:

• To develop and demonstrate a novel beta-cyclodextrin (β-CD) based optic-fiber microfiber biosensor.
• To enable rapid and sensitive detection of cholesterol concentration.
• To evaluate the sensor's performance characteristics, including sensitivity and selectivity.

Main Methods:

• Immobilization of β-cyclodextrin on an optic-fiber microfiber surface.
• Utilizing the principle of refractive index (RI) change upon cholesterol complexation.
• Translating RI changes into wavelength shifts in the interference spectrum.
• Characterization using infrared spectroscopy.

Main Results:

• The biosensor demonstrated high RI sensitivity (1251 nm/RIU) and low-temperature sensitivity (-0.019 nm/°C).
• Rapid cholesterol detection was achieved in the concentration range of 0.001 to 1 mM.
• A sensitivity of 12.7 nm/(mM) was observed in the low concentration range (0.001 to 0.05 mM).
• Infrared spectroscopy confirmed the sensor's ability to detect cholesterol.

Conclusions:

• The proposed β-CD based optic-fiber biosensor is effective for cholesterol detection.
• The sensor exhibits high sensitivity and good selectivity.
• This technology holds significant potential for various biomedical applications requiring cholesterol monitoring.