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Updated: Jul 28, 2025

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
Published on: December 16, 2016
Microplastics in surface water of the Bay of Asunción, Paraguay
Diana Beatriz Diez-Pérez1, Inés Arenas2, Erenia Maidana2
1National University of Asunción, Faculty of Chemical Sciences, Department of Physical Chemistry, San Lorenzo, Paraguay; University of A Coruña, Group of Applied Analytical Chemistry, Institute of Environmental Science (IUMA), Campus da Zapateira, 15071 A Coruña, Spain.
Abstract:
This study identified and quantified microplastics in the Bay of Asunción, Paraguay, and its main tributaries. Surface water samples were sieved in duplicate at six locations using stainless-steel sieves (0.3-4.75 mm range), digested employing the Fenton's reaction (Fe-catalysed H2O2 digestion), and floated using NaCl and NaI. Particles were inspected using a microscope and characterized by IR spectrometry. Microplastics were found in all samples; more abundant (p < 0.05) in water from the bay (13.2 ± 13.4 items·m-3) than from the tributaries (1.0 ± 0.5 items·m-3). Most microplastics were common polymers and their abundance was in the order polypropylene > high-density polyethylene > low-density polyethylene, transparent and white. The results were similar to other regional studies and suggested that their main source was single-use packaging, disposed inadequately due to poor garbage collection.
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