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Related Experiment Video

Updated: Jul 28, 2025

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

Digital spatial profiling of CD4

Mai Takeuchi¹, Hiroaki Miyoshi¹, Yuichiro Semba²

¹Department of Pathology, Kurume University School of Medicine, Kurume City, Fukuoka, Japan.

Virchows Archiv : an International Journal of Pathology

|June 3, 2023

Summary

This summary is machine-generated.

Classic Hodgkin lymphoma (CHL) features CD4+ T cell rosettes interacting with tumor cells. These rosettes show higher immune checkpoint molecule expression, offering new insights into the CHL tumor microenvironment.

Keywords:

CD4+ T cell rosettes Classic Hodgkin lymphoma Digital spatial profiling Hodgkin-Reed-Sternberg cells Tumor microenvironment

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Published on: September 13, 2022

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Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Published on: January 7, 2019

Area of Science:

Immunology
Oncology
Pathology

Background:

Classic Hodgkin lymphoma (CHL) is characterized by rare malignant Hodgkin-Reed-Sternberg (HRS) cells within a rich inflammatory infiltrate.
CD4+ T cells form distinct rosettes around HRS cells, suggesting a crucial role in the tumor microenvironment (TME).

Purpose of the Study:

To investigate the gene expression profiles of CD4+ T cell rosettes surrounding HRS cells in CHL.
To elucidate the functional interactions between HRS cells and CD4+ T cells within the CHL TME.

Main Methods:

Digital spatial profiling was employed to compare gene expression between CD4+ T cell rosettes and other CD4+ T cells.
Immunohistochemistry was used to validate the expression of key immune checkpoint molecules.

Main Results:

CD4+ T cell rosettes exhibited significantly higher expression of immune checkpoint molecules, including OX40, programmed cell death-1 (PD-1), and cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), compared to other CD4+ T cells.
Immunohistochemical analysis confirmed the variable expression of PD-1, CTLA-4, and OX40 within these CD4+ T cell rosettes.

Conclusions:

This study introduces a novel pathological approach for analyzing the CHL TME.
The findings provide deeper insights into the specific role and immune status of CD4+ T cells in close proximity to HRS cells in CHL.