Related Experiment Video
Updated: Jul 28, 2025
08:27
Design and Validation of a Volumetric-extrusion Bioprinter for Bioprinting of Soluble Basement Membrane Extract for Translational Research
Published on: March 28, 2025
290
m
Xiaolong Zhu1,2,3,4, Xingwei Wu1,2,3, Hui Yang1,2,3,4
1Central Laboratory, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, 241001, People's Republic of China.
Biology Direct
|June 3, 2023
Summary
This study reveals that the long non-coding RNA LINC01003 promotes glioma progression by regulating the focal adhesion pathway. Targeting LINC01003 offers a potential therapeutic strategy for glioma patients.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNAs (lncRNAs) are implicated in glioma development.
- The specific role and regulatory mechanisms of LINC01003 in glioma remain largely uncharacterized.
Purpose of the Study:
- To investigate the functional significance of LINC01003 in glioma.
- To elucidate the molecular mechanisms underlying LINC01003's role in glioma progression.
Main Methods:
- Utilized GEPIA2 and Chinese Glioma Genome Atlas (CCGA) databases for expression and survival analysis.
- Performed in vitro and in vivo loss-of-function experiments to assess LINC01003's impact on glioma.
- Employed RNA sequencing, bioinformatics, and RNA immunoprecipitation (RIP) assays to identify pathways and regulatory mechanisms, including N6-methyladenine (m6A) modification.
Main Results:
- LINC01003 expression is significantly upregulated in glioma tissues and cell lines.
- Elevated LINC01003 levels correlate with poorer overall survival in glioma patients.
- Knockdown of LINC01003 suppressed glioma cell cycle, proliferation, and migration, mediated via the focal adhesion signaling pathway.
- LINC01003 upregulation is dependent on m6A modification regulated by METTL3.
Conclusions:
- LINC01003 acts as a oncogenic lncRNA promoting glioma tumorigenesis.
- The LINC01003-CAV1-FAK axis represents a promising therapeutic target for glioma treatment.
More Related Videos
Related Concept Videos
01:54Molar Mass
70.7K
The identity of a substance is defined not only by the types of atoms or ions it contains but by the quantity of each type of atom or ion. For example, water, H2O, and hydrogen peroxide, H2O2, are alike in that their respective molecules are composed of hydrogen and oxygen atoms. However, because a hydrogen peroxide molecule contains two oxygen atoms, as opposed to the water molecule, which has only one, the two substances exhibit very different properties.
70.7K
01:19Thomson's e/m Experiment
3.9K
In a beam of charged particles created by a heated cathode, the particles move at different speeds. However, many applications need a beam with uniform particle speeds. An arrangement known as a velocity selector uses electric and magnetic fields to pick particles with a particular speed from the beam.
A particle with charge q, speed v, and mass m enters an area from the top, where the magnetic and electric fields are perpendicular both to the particle's motion and to one another. The...
A particle with charge q, speed v, and mass m enters an area from the top, where the magnetic and electric fields are perpendicular both to the particle's motion and to one another. The...
3.9K
01:22Motional Emf
3.3K
Magnetic flux depends on three factors: the strength of the magnetic field, the area through which the field lines pass, and the field's orientation with respect to the surface area. If any of these quantities vary, a corresponding variation in magnetic flux occurs. If the area through which the magnetic field lines are passing changes, then the magnetic flux also changes. This change in the area can be of two types: the flux through the rectangular loop increases as it moves into the...
3.3K
01:21Masonry in Cold and Hot Weather Conditions
114
In cold weather, masonry construction requires specific precautions to ensure mortar does not freeze before curing, as this can significantly weaken its strength and watertightness. Mortar temperature should be maintained between 60°F and 80°F to support proper hydration and curing. Below 40°F, mortar water must be heated, but should not exceed 120°F as high temperatures can reduce mortar's compressive and bond strength.
Other key practices include keeping masonry units...
Other key practices include keeping masonry units...
114
01:13Mass Moment of Inertia: Problem Solving
346
Knowing how to determine the moment of inertia in a wheel's axle can be invaluable in engineering and automotive applications. It provides an understanding of how changes in geometry, mass, and radius can impact its performance.
The axle can be approximated to a solid cylinder with longitudinal and perpendicular axes. Initially, a thin disc is considered parallel to the circular face of the cylinder.
The axle can be approximated to a solid cylinder with longitudinal and perpendicular axes. Initially, a thin disc is considered parallel to the circular face of the cylinder.
346
01:22Thermal Insulation in Masonry Walls
154
In hot, dry climates, the thermal mass of masonry walls can be beneficial, absorbing heat during the day and releasing it at night, thereby stabilizing indoor temperatures. However, in most other climates, additional insulation is necessary to enhance thermal resistance.
External insulation can be applied using an Exterior Insulation and Finish System (EIFS), which involves affixing panels of plastic foam to the wall and covering them with a polymeric stucco reinforced with glass fiber mesh....
External insulation can be applied using an Exterior Insulation and Finish System (EIFS), which involves affixing panels of plastic foam to the wall and covering them with a polymeric stucco reinforced with glass fiber mesh....
154