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Related Experiment Video

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Protocol to prepare mouse spinal cord for patch-clamp and histology experiments.

Sang-Hyeon Ju1, Jong-Woo Sohn2

  • 1Department of Internal Medicine, Chungnam National University Hospital, Daejeon 35015, South Korea.

STAR Protocols
|June 4, 2023
PubMed
Summary

This study details a protocol for preparing mouse spinal cord tissue for patch-clamp electrophysiology and histology. Researchers can now assess sympathetic preganglionic neuron activity and protein expression more effectively.

Keywords:
MetabolismNeuroscience

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Anatomy

Background:

  • The central nervous system includes the spinal cord, housed within the vertebral spinal canal.
  • Understanding spinal cord function requires robust experimental preparation methods.

Purpose of the Study:

  • To present a detailed protocol for preparing mouse spinal cord sections.
  • To enable patch-clamp and histology experiments on sympathetic preganglionic neurons.

Main Methods:

  • Isolation of the mouse spinal cord from the spinal canal.
  • Preparation of acute spinal cord slices for patch-clamp recordings.
  • Fixation of spinal cord tissue for cryosectioning and imaging.

Main Results:

  • The protocol facilitates the assessment of neuronal activity in spinal cord sections.
  • The protocol allows for the evaluation of protein expression in sympathetic preganglionic neurons.

Conclusions:

  • This protocol provides a standardized method for spinal cord tissue preparation.
  • It supports research into the function and molecular characteristics of sympathetic preganglionic neurons.