Related Experiment Video
Updated: Jul 28, 2025
10:25
Easy and Accurate Mechano-profiling on Micropost Arrays
Published on: November 17, 2015
11.2K
Profiling
Nurul Najwa Ainaa Alias1, Sharina Omar1, Nur Indah Ahmad1
1Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Journal of Veterinary Science
|June 4, 2023
Summary
Feline Bartonella infection is common in shelter cats, with flea infestation being a significant risk factor. This highlights potential risks for transmission to humans, emphasizing the need for better disease management in shelters.
Area of Science:
- Veterinary Medicine
- Infectious Diseases
- Public Health
Background:
- Shelter animals face increased disease risk due to management and vector control challenges.
- Feline Bartonella infection, caused by Bartonella henselae, is a zoonotic disease transmitted by vectors.
- Shelter environments can facilitate the spread of vector-borne diseases like Bartonella.
Purpose of the Study:
- To investigate Bartonella infection prevalence in shelter cats using molecular and serological methods.
- To profile the CD4:CD8 immune cell ratio in infected cats.
- To identify risk factors associated with Bartonella infection in this population.
Main Methods:
- Polymerase chain reaction (PCR) and DNA sequencing for Bartonella DNA detection.
- Indirect immunofluorescence assay for Bartonella IgM and IgG antibody detection.
- Flow cytometry for CD4:CD8 T-cell ratio analysis.
Main Results:
- Bartonella henselae and B. clarridgeiae were detected in oral swabs and cat fleas.
- A high seroprevalence of Bartonella henselae was observed, with no antibody-free cats in any shelter.
- Flea infestation was identified as the sole significant risk factor for Bartonella infection.
Conclusions:
- This study is the first to compare Bartonella spp. antigen, antibody status, and CD4:CD8 ratios in shelter cats.
- High seropositivity suggests potential for undetectable infections and human transmission.
- Effective flea control is crucial for managing Bartonella in shelters and preventing zoonotic spread.
Related Concept Videos
01:26Profile Leveling and Cross Sections
351
Profile leveling and cross-sections are surveying methods used to determine and document terrain elevations for infrastructure projects such as highways, railroads, canals, and pipelines. These methods provide data for earthwork planning and alignment of proposed routes. Profile leveling involves measuring elevations along a fixed line to create a vertical terrain profile. A surveyor sets up a leveling instrument at the benchmark (BM) and records a backsight (BS) to determine the...
351
02:24Ribosome Profiling
3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
03:46Qualitative Analysis
22.4K
For solutions containing mixtures of different cations, the identity of each cation can be determined by qualitative analysis. This technique involves a series of selective precipitations with different chemical reagents, each reaction producing a characteristic precipitate for a specific group of cations. Metal ions within a group are further separated by varying the pH, heating the mixture to redissolve a precipitate, or adding other reagents to form complex ions.
For instance, group IV...
For instance, group IV...
22.4K
01:12Taping Over Different Ground Profiles
54
Taping over varying ground profiles requires careful adaptation to achieve accurate measurements. On smooth, level ground with minimal vegetation, the tape can rest directly on the ground. Here, the taping team, typically consisting of a head and a rear tapeman, coordinates their positions with clear communication. The rear tapeman holds the tape at the starting point and guides the head tapeman toward a range pole placed beyond the endpoint, using hand or voice signals to ensure alignment.On...
54
02:13The Representativeness Heuristic
15.8K
The representative heuristic describes a biased way of thinking, in which you unintentionally stereotype someone or something. For example, you may assume that your professors spend their free time reading books and engaging in intellectual conversation, because the idea of them spending their time playing volleyball or visiting an amusement park does not fit in with your stereotypes of professors.
15.8K
01:22Self-Report Tests of Personality
394
Self-report inventories are objective personality assessments that use multiple-choice items or numbered scales, typically ranging from 1 (strongly disagree) to 5 (strongly agree). They are often called Likert scales after Rensis Likert. These inventories are widely used due to their ease of administration and cost-effectiveness. One of the most prominent examples is the Minnesota Multiphasic Personality Inventory (MMPI), initially developed in the 1940s to assess abnormal personality traits.
394