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High-Strength Cell Sheets and Vigorous Hydrogels from Mesenchymal Stem Cells Derived from Human Embryonic Stem Cells
Baojie Guo1,2,3, Yongchao Duan1,2, Zhongwen Li1,2,4
1State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
ACS Applied Materials & Interfaces
|June 5, 2023
Summary
Researchers developed a novel cell sheet platform using human embryonic stem cell-derived cells. This platform offers enhanced mechanical strength and therapeutic potential for tissue engineering and regenerative medicine applications.
Area of Science:
- Stem cell biology
- Tissue engineering
- Regenerative medicine
Background:
- Natural cell derivatives like cell sheets (CSs) and matrix gels are crucial in tissue engineering.
- Current methods face limitations due to heterogeneity and poor mechanical properties of CSs and hydrogels.
Purpose of the Study:
- To develop a long-term serum-free culture system for human embryonic stem cell (hESC)-derived immunity-and-matrix-regulatory cells (IMRCs).
- To engineer high-strength cell sheets (CSs) and injectable hydrogels for regenerative medicine.
Main Methods:
- Established a 21-day long-term serum-free culture system for hESC-derived IMRCs.
- Characterized the mechanical properties and extracellular matrix (ECM) secretion of IMRC-CSs.
- Investigated the therapeutic potential of IMRC-CSs in corneal injury repair and uterine adhesion repair via decellularized hydrogels.
Main Results:
- IMRC-CSs exhibited significantly enhanced ECM secretion and mechanical properties compared to umbilical cord-derived MSCs.
- Demonstrated a ten-thousand-fold increase in elastin, a higher elastic modulus (1500 kPa), thicker structure (20.59 μm), and higher fiber density.
- Showcased IMRC-CSs' efficacy in promoting corneal chemical injury repair and their conversion into injectable hydrogels for uterine adhesion repair.
Conclusions:
- Successfully established a high-strength cell sheet platform using human pluripotent stem cells for the first time.
- The developed platform provides a facile and scalable engineering approach for regenerative medicine.
- IMRC-CSs hold significant promise for therapeutic applications in tissue repair and regeneration.
