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Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
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Quaking regulates circular RNA production in cardiomyocytes.
Pablo Montañés-Agudo1, Ingeborg van der Made1, Simona Aufiero1
1Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Amsterdam UMC location University of Amsterdam, 1105AZ, Amsterdam, The Netherlands.
Journal of Cell Science
|June 5, 2023
Summary
The RNA-binding protein quaking (QKI) influences circular RNA (circRNA) production in the heart. Loss of QKI impacts circRNA expression and reveals links between linear splicing and circRNA formation.
Area of Science:
- Molecular Biology
- Genetics
- Cardiovascular Research
Background:
- Circular RNAs (circRNAs) are non-coding RNA molecules implicated in diverse pathophysiological processes.
- The RNA-binding protein quaking (QKI) is known to regulate circRNA biogenesis.
Purpose of the Study:
- To investigate the role of QKI in cardiac circRNA formation.
- To understand the relationship between QKI-dependent splicing and circRNA production.
Main Methods:
- RNA-sequencing was performed on Qki-knockout mice to analyze circRNA expression.
- QKI-regulated circRNAs were compared with those regulated by RBM20.
Main Results:
- Loss of QKI led to differential expression of 17% of circRNAs in adult mouse hearts.
- 58% of QKI-regulated circRNAs originated from genes with QKI-dependent splicing.
- QKI and RBM20 regulate distinct yet overlapping sets of cardiac circRNAs, with some Ttn-derived circRNAs showing opposing regulation.
Conclusions:
- QKI plays a significant role in regulating alternative splicing and circRNA formation in the heart.
- QKI and RBM20 exhibit partially overlapping but distinct regulatory roles in cardiac circRNA biogenesis.
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