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Complete Genome Sequence of Invasive MLST-Untypeable Serotype 3
Sung Guk Kim1, Christine V Summage-West1, Mariela Reyna1
1Surveillance/Diagnostic Laboratory, Office of Scientific Coordination, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas, USA.
Abstract:
Serotype 3 Streptococcus pneumoniae is associated with major invasive pneumococcal diseases in humans. We report the circularized 2.0-Mbp complete genome sequence of invasive serotype 3 S. pneumoniae strain B1900, untypeable by multilocus sequence typing (MLST). This strain was isolated from the brain of an infant rhesus monkey (Macaca mulatta) that suddenly died of meningitis with no clinical symptoms.
Insights
We sequenced the complete genome of an invasive serotype 3 Streptococcus pneumoniae strain from a rhesus monkey. This provides insights into the genetic makeup of this important human pathogen.
Area of Science:
- Microbiology
- Genomics
- Pathogen Research
Background:
- * Serotype 3 Streptococcus pneumoniae is a significant cause of invasive pneumococcal diseases in humans.
- * Invasive pneumococcal disease (IPD) poses a substantial global health burden.
- * Understanding the genomic characteristics of S. pneumoniae is crucial for developing effective interventions.
Purpose of the Study:
- * To report the complete genome sequence of an invasive serotype 3 Streptococcus pneumoniae strain, B1900.
- * To characterize an MLST-untypeable S. pneumoniae strain isolated from a non-human primate.
- * To provide genomic data for studying pneumococcal pathogenesis and evolution.
Main Methods:
- * Whole-genome sequencing of Streptococcus pneumoniae strain B1900.
- * Genome assembly and annotation.
- * Multilocus sequence typing (MLST) analysis.
Main Results:
- * The complete, circularized genome sequence of S. pneumoniae strain B1900 was determined to be 2.0 Mbp.
- * Strain B1900 is an invasive serotype 3 isolate.
- * The strain was found to be untypeable by standard MLST protocols.
Conclusions:
- * The genome sequence of invasive serotype 3 S. pneumoniae strain B1900 provides a valuable resource for research.
- * Characterization of this strain, isolated from a rhesus monkey, may offer insights into zoonotic potential or broader pneumococcal epidemiology.
- * Further studies are warranted to explore the implications of MLST untypeability and the genomic features of this invasive strain.
