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Related Experiment Video

Updated: Jul 28, 2025

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
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Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

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Characterisation of acetogen formatotrophic potential using Eubacterium limosum.

Jamin C Wood1, R Axayacatl Gonzalez-Garcia2, Dara Daygon2,3

  • 1Australian Centre for Water and Environmental Biotechnology (ACWEB), The University of Queensland, QLD, Brisbane, 4072, Australia.

Applied Microbiology and Biotechnology
|June 5, 2023
PubMed
Summary

Eubacterium limosum utilizes formate for growth, but most carbon becomes CO2, not acetate. This formate metabolism involves protein expression changes and pyruvate accumulation, indicating cellular stress.

Keywords:
AcetogenChemostatEubacterium limosumFormateMetabolomicsProteomics

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Area of Science:

  • Microbiology
  • Metabolic Engineering
  • Biotechnology

Background:

  • Formate is a potential energy carrier for renewable electricity.
  • Acetogenic bacteria like Eubacterium limosum can use formate for growth, attracting biotechnological interest.
  • Formatotrophic metabolism in E. limosum is not well understood, lacking system-level data from continuous cultures.

Purpose of the Study:

  • To provide the first steady-state omics dataset for Eubacterium limosum growing on formate.
  • To characterize formatotrophic metabolism in E. limosum at a system level.
  • To investigate the metabolic and protein expression responses to formate utilization.

Main Methods:

  • Continuous culture cultivation of E. limosum.
  • Measurement of formate uptake and carbon dioxide production rates.
  • Differential protein expression analysis.
  • Intracellular metabolomics.

Main Results:

  • High specific formate uptake rate (280 ± 56 mmol/gDCW/d) was observed at a dilution rate of 0.4 d-1.
  • The majority of consumed formate carbon was converted to CO2 (150 ± 11 mmol/gDCW/d), not acetate.
  • Protein upregulation of phosphotransacetylase (Pta) and pyruvate formate ligase (Pfl) was observed, alongside intracellular pyruvate accumulation, suggesting energy limitation and stress.

Conclusions:

  • Formatotrophic growth in E. limosum is characterized by high formate uptake but significant carbon loss as CO2.
  • Metabolic regulation occurs at the protein expression level, with Pta and Pfl playing key roles.
  • Formate may induce intracellular stress and potentially lead to biofilm formation in E. limosum.
  • Formate could be a valuable one-carbon substrate for producing pyruvate-rich chemicals.