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Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

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Therapeutic potential of

Md Huzzatul Mursalin¹, Roger Astley², Phillip S Coburn^1,2

¹Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.

|June 5, 2023

Summary

This summary is machine-generated.

Bacteriophage lysins like PlyB offer a novel treatment for Bacillus cereus eye infections, effectively killing bacteria and preventing vision loss. This phage lysin shows promise as a safe and potent alternative to antibiotics.

Keywords:

Bacillus bacteriophage lysin endophthalmitis keratitis ocular infection

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Area of Science:

Microbiology
Ophthalmology
Biotechnology

Background:

Antimicrobial resistance necessitates novel therapeutic strategies.
Bacillus cereus causes severe, blinding intraocular infections resistant to conventional antibiotics.
Phage lysins are emerging as a potent alternative to antibiotics.

Purpose of the Study:

To evaluate the efficacy and safety of the bacteriophage lysin PlyB against Bacillus cereus ocular infections.
To determine PlyB's activity against vegetative B. cereus and its spores.
To assess PlyB's therapeutic potential in experimental models of bacterial keratitis and endophthalmitis.

Main Methods:

In vitro testing of PlyB against vegetative B. cereus and spores.
Assessment of PlyB's specificity and activity in ex vivo rabbit vitreous.
Evaluation of PlyB's cytotoxicity and hemolytic activity.
In vivo studies using intravitreal and topical administration in experimental ocular infection models.

Main Results:

PlyB rapidly killed vegetative B. cereus but not its spores in vitro.
PlyB demonstrated high group specificity and efficacy under various conditions, including ex vivo vitreous.
No cytotoxic or hemolytic activity was observed; no innate activation was triggered.
Intravitreal and topical PlyB administration effectively treated experimental B. cereus keratitis and endophthalmitis, preventing tissue damage.

Conclusions:

PlyB is a safe and effective therapeutic agent for Bacillus cereus ocular infections.
Phage lysin PlyB shows significant potential for treating blinding eye infections caused by antibiotic-resistant bacteria.
This study establishes PlyB as a promising alternative to conventional antibiotics for B. cereus-related ophthalmic conditions.