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Related Experiment Videos

Daniel Nemeth1, Numana Luqman2, Loretta Chen3

  • 1Stiles-Nicholson Brain Institute, College of Medicine, Florida Atlantic University, Boca Raton, Florida, United States.

Micropublication Biology
|June 5, 2023
PubMed
Summary

The Aldh1l1-Cre/ER T2 mouse model shows unexpected Cre recombinase activity in spleen and exocrine glands, not just astrocytes. This finding is crucial for researchers using this transgenic mouse for central nervous system studies.

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Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • The Aldh1l1-Cre/ER T2 mouse model is utilized for conditional Cre recombinase expression in central nervous system astrocytes.
  • The specificity of Aldh1l1 promoter-driven Cre activity outside of astrocytes has not been previously reported.

Purpose of the Study:

  • To investigate potential off-target Cre recombinase activity of the Aldh1l1-Cre/ER T2 mouse model.
  • To identify cell populations expressing Cre recombinase under the Aldh1l1 promoter.

Main Methods:

  • Generation of a reporter mouse line by crossing Aldh1l1-Cre/ER T2 mice with ROSA26:CAG-LSL-TdTomato reporter mice.
  • Gross anatomical observation and histological analysis to detect TdTomato expression, indicating Cre activity.

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Main Results:

  • Significant TdTomato expression was observed in the spleen and exocrine glands, including the salivary glands (parotid, submandibular, sublingual) and pancreas.
  • TdTomato expression specifically targeted serous cells in these glands and fibroblast-like cells in the spleen and submandibular lymph nodes.
  • This indicates unintended Cre recombinase activity driven by the Aldh1l1 promoter in non-astrocyte populations.

Conclusions:

  • The Aldh1l1-Cre/ER T2 mouse model exhibits Cre recombinase activity in exocrine glands and spleen, beyond its intended astrocyte-specific expression.
  • These findings highlight the need for careful consideration of this model's activity patterns in research.
  • Potential implications for biological and behavioral data interpretation in studies utilizing this transgenic mouse model.