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QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
Published on: June 22, 2017
A QTL on chromosome IV explains a natural variation of QR.pap final position in
Clément Dubois1, Marie-Anne Félix1
1Institut de Biologie de l'École Normale Supérieure, Paris, Île-de-France, France.
Abstract:
In Caenorhabditis elegans , the QR neuroblast and its progeny migrate from the posterior to the anterior part of the animal during the L1 stage. We previously showed that the final position of QR.pa daughters varies among C. elegans wild isolates, with CB4932 displaying a particularly anterior QR.pap position (Dubois et al., 2021). Here, we study the genetic basis of the variation between isolates CB4932 and JU1242. We show that JU1242 alleles behave in a mostly dominant fashion. Using a Bulk Segregant Analysis, we detect a quantitative trait locus (QTL) region on chromosome IV. This QTL was confirmed using reciprocal chromosome IV introgressions.
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