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Updated: Jul 27, 2025

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UHPLC-QTOF-MS Metabolic Profiling of

Ayesha Asif1, Saiqa Ishtiaq1, Sairah Hafeez Kamran2

  • 1Punjab University College of Pharmacy, University of the Punjab, Lahore 54000, Pakistan.

ACS Omega
|June 5, 2023
PubMed
Summary

Marchantia polymorpha extract demonstrates significant hepatoprotective effects against paracetamol-induced liver damage in mice. This traditional remedy shows promise for treating liver toxicity by restoring biochemical markers and liver architecture.

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Area of Science:

  • Pharmacology
  • Hepatology
  • Phytochemistry

Background:

  • Traditional medicine often utilizes plant-derived compounds for therapeutic purposes.
  • Liver failure and hepatotoxicity pose significant global health challenges.
  • Marchantia species have a history of use in treating liver ailments.

Purpose of the Study:

  • To investigate the hepatoprotective potential of Marchantia polymorpha chloroform extract.
  • To evaluate the efficacy of the extract in a mouse model of paracetamol-induced liver injury.
  • To identify potential bioactive compounds responsible for the observed hepatoprotective activity.

Main Methods:

  • Induction of liver damage in mice using paracetamol.
  • Administration of Marchantia polymorpha chloroform extract at varying doses.
  • Biochemical analysis of liver enzymes, bilirubin, lipids, and antioxidant markers.
  • Histopathological examination of liver tissues.
  • Metabolic profiling using ultrahigh performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS).

Main Results:

  • The extract significantly reduced liver enzymes (AST, ALT, ALP) and bilirubin levels.
  • It normalized lipid profiles, including cholesterol and triglycerides, and increased HDL levels.
  • Antioxidant enzyme activity (superoxide dismutase, glutathione peroxidase) was elevated, and lipid peroxidation was reduced.
  • Histopathology revealed restored liver architecture and reduced sinusoidal damage.
  • Metabolomic analysis tentatively identified 28 compounds, including phenols, terpenoids, and lipids, potentially contributing to the hepatoprotection.

Conclusions:

  • Marchantia polymorpha chloroform extract exhibits significant dose-dependent hepatoprotective activity.
  • The extract effectively mitigates paracetamol-induced liver damage by improving biochemical markers and antioxidant status.
  • This study validates the traditional use of Marchantia species for liver health and identifies key compounds for further investigation.