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Related Experiment Video

Updated: Jul 27, 2025

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

Microfluidic fabrication of stable collagen microgels with aligned microstructure using flow-driven co-deposition and

Santiago O Correa¹, Xiaolong Luo^2,3, Christopher B Raub^1,3

¹Department of Biomedical Engineering, Washington DC, United States of America.

Journal of Micromechanics and Microengineering : Structures, Devices, and Systems

|June 5, 2023

Summary

Researchers developed a microfluidic method to create stable, aligned collagen hydrogels. This technique offers precise control over gel thickness and structure, advancing on-chip tissue models and drug delivery applications.

Keywords:

alginate birefringence collagen extracellular matrix microfluidics microgels orientation spatial

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Last Updated: Jul 27, 2025

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Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

Published on: September 29, 2015

Area of Science:

Biomaterials Engineering
Microfluidics
Tissue Engineering

Background:

Physiologically accurate on-chip tissue models require stable, aligned collagen hydrogels.
Current methods face challenges in controlling hydrogel formation and stability within microfluidic devices.

Purpose of the Study:

To develop a controlled biofabrication method for stable, aligned collagen hydrogels in microfluidic devices.
To enable the creation of advanced on-chip models for tissue and organ research.

Main Methods:

Collagen-alginate microgels were formed via calcium crosslinking in a microfluidic channel.
Chitosan membranes controlled ion diffusion, enabling rapid, self-limiting gel formation.
Gel thickness was tuned by adjusting calcium concentration and flow rate.

Main Results:

Achieved controlled gel thickness (30-200 μm) by balancing diffusion and convection.
Demonstrated stable, aligned collagen networks using birefringence and texture analysis.
Confirmed size-dependent dextran diffusion, indicating construct accessibility for molecules.

Conclusions:

Physicochemical parameters precisely control collagen gel formation in microfluidics.
This method supports on-chip models for studying extracellular matrix invasion, cancer, and drug delivery.