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Updated: Jul 27, 2025
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Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
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Advances in sequencing-based studies of microDNA and ecDNA: Databases, identification methods, and integration with
Rong Jiang1, Manqiu Yang1, Shufan Zhang1
1School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China.
Computational and Structural Biotechnology Journal
|June 5, 2023
Summary
Extrachromosomal circular DNA (eccDNA) and large extrachromosomal DNA (ecDNA) are crucial in cancer. This review covers their mechanisms, applications, and advanced detection methods like single-cell analysis.
Area of Science:
- Genetics
- Molecular Biology
- Cancer Research
Background:
- Extrachromosomal circular DNA (eccDNA) are DNA molecules separate from chromosomes, found in many organisms.
- Large extrachromosomal DNA (ecDNA) are prevalent in cancer cells and drive cancer development, progression, evolution, and drug resistance.
Purpose of the Study:
- To review advances in the biological mechanisms and applications of microDNA and ecDNA.
- To summarize current identification tools for eccDNA using next-generation (NGS) and third-generation sequencing (TGS).
- To highlight recent integration of ecDNA with single-cell analysis and suggest future directions.
Main Methods:
- Review of current scientific literature on eccDNA and ecDNA.
- Analysis of next-generation (NGS) and third-generation sequencing (TGS) techniques for eccDNA identification.
- Examination of single-cell analysis integration with ecDNA research.
Main Results:
- eccDNA, particularly large ecDNA, plays a significant role in cancer biology.
- NGS and TGS are key technologies for identifying eccDNA across the genome.
- Integration with single-cell analysis offers new insights into ecDNA heterogeneity and function.
Conclusions:
- Understanding eccDNA mechanisms and applications is vital for cancer research.
- Advanced sequencing and single-cell technologies are improving eccDNA detection and analysis.
- Further research integrating these methods will advance our comprehension of cancer.
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