Melatonin Promotes BMSCs Osteoblastic Differentiation and Relieves Inflammation by Suppressing the NF-

Area of Science:

• Cell Biology
• Endocrinology
• Orthopedics

Background:

• Bone mesenchymal stem cells (BMSCs) are crucial for bone metabolism.
• Reduced osteogenic function of BMSCs is linked to osteoporosis.
• Melatonin is a key hormone regulating physiological processes.

Purpose of the Study:

• To investigate melatonin's effect on BMSC osteoblastic differentiation.
• To elucidate the mechanisms underlying melatonin's action.

Main Methods:

• Isolation of rat BMSCs.
• Assessment of osteogenic differentiation using alizarin red and ALP staining.
• Analysis of osteogenic gene expression (ALP, Col 1, OCN, OPN, RUNX2).
• Investigation of the NF-κB signaling pathway and TNF-α effects.

Main Results:

• Melatonin significantly improved BMSC osteogenic differentiation.
• Melatonin upregulated key osteogenic genes.
• Melatonin suppressed the inflammatory response by inhibiting the NF-κB pathway.
• Melatonin reversed TNF-α-induced inhibition of osteogenesis and inflammation.

Conclusions:

• Melatonin promotes osteoblastic differentiation of BMSCs.
• Melatonin exerts anti-inflammatory effects via the NF-κB pathway.
• Melatonin demonstrates therapeutic potential for osteoporosis treatment.