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Related Experiment Videos

Laurine Kaul1,2,3, Adrian I Abdo1,3, Tom Coenye4

  • 1Richter Lab, Department of Surgery, Basil Hetzel Institute for Translational Health Research, University of Adelaide, 37 Woodville Road, Adelaide, SA, 5011, Australia.

Biofilm
|June 5, 2023
PubMed
Summary

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This summary is machine-generated.

A novel copper complex, Cu(DDC)2 plus Cu2+, effectively combats Staphylococcus biofilms, a common cause of surgical site infections. A liposomal formulation offers improved solubility and efficacy for future wound treatment applications.

Area of Science:

  • Biochemistry and Microbiology
  • Materials Science
  • Drug Delivery Systems

Background:

  • Surgical site infections (SSIs) are frequently caused by Staphylococcus aureus and Staphylococcus epidermidis biofilms, which are resistant to conventional antibiotics.
  • The development of antibiotic resistance necessitates alternative therapeutic strategies to effectively treat biofilm-associated infections.

Purpose of the Study:

  • To evaluate the in vitro efficacy of a copper diethyldithiocarbamate (Cu(DDC)2) complex with additional Cu2+ against Staphylococcus biofilms.
  • To assess the antibacterial and antibiofilm activity of a liposomal formulation of Cu(DDC)2 + Cu2+.

Main Methods:

  • In vitro assessment of Cu(DDC)2 + Cu2+ against S. aureus and S. epidermidis biofilms on hernia mesh and in a wound model using colony forming unit (CFU) counting.
Keywords:
BiofilmsCopper ionsDiethyldithiocarbamateLiposomesStaphylococcus aureusStaphylococcus epidermidisSurgical site infections

Related Experiment Videos

  • Preparation of liposomal Cu(DDC)2 and Cu2+ formulations.
  • In vitro (alamarBlue assay, CFU counting) and in vivo (Galleria mellonella infection model) evaluation of liposomal formulations.

    • Main Results:

    • A combination of 35 μM DDC- and 128 μM Cu2+ significantly inhibited S. aureus and S. epidermidis biofilms in vitro.
    • Liposomal Cu(DDC)2 + Cu2+ demonstrated comparable antibiofilm activity to the non-liposomal form and enhanced survival in a Galleria mellonella model.
    • The liposomal formulation exhibited good in vitro antibiofilm activity, in vivo antibacterial efficacy, and low toxicity in the insect model.

    Conclusions:

    • The Cu(DDC)2 + Cu2+ complex shows significant in vitro antibiofilm activity against clinically relevant Staphylococcus biofilms.
    • The liposomal formulation of Cu(DDC)2 + Cu2+ is a promising, water-soluble alternative for treating biofilm infections, with potential for future in vivo applications.