Related Experiment Video
Updated: Jul 27, 2025
10:34
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Published on: March 15, 2019
22.9K
Complete genome analysis of
Maryoris Elisa Soto Lopez1,2, Marco Tulio Pardini Gontijo1,3, Rodrigo Rezende Cardoso1
1Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Frontiers in Cellular and Infection Microbiology
|June 5, 2023
Summary
The bacteriophage Tequatrovirus ufvareg1 (UFV-AREG1) shows potential for controlling Escherichia coli O157:H7 in food. Its genome analysis reveals conserved features within the Tequatrovirus genus, supporting its biocontrol applications.
Area of Science:
- Microbiology
- Genomics
- Food Safety
Background:
- Bacteriophages are explored as biocontrol agents against human pathogens.
- Understanding phage genetics is crucial for safe application in the food industry.
- Tequatrovirus ufvareg1 (UFV-AREG1) was previously shown to inhibit Escherichia coli O157:H7.
Purpose of the Study:
- To characterize the genome of the bacteriophage T. ufvareg1 (UFV-AREG1).
- To compare the T. ufvareg1 genome with other Tequatrovirus species.
- To assess the potential of T. ufvareg1 as a biocontrol agent for food safety.
Main Methods:
- Genome sequencing of T. ufvareg1 using Illumina HiSeq.
- Analysis of viral morphology, latent period, and burst size.
- Genomic comparison using VIRIDIC and VIPTree, including pan-genomic analysis.
Main Results:
- T. ufvareg1 exhibits specific virion morphology and a latent period of 25 min with a burst size of 18.
- The genome contains 268 coding DNA sequences (CDS), 10 tRNA genes, 40 promoters, and 2 terminators.
- High genomic similarity (88.77% VIRIDC, 88.91% VipTree) was observed with other Tequatrovirus, indicating conserved genomes with core, softcore, shell, and cloud genes.
Conclusions:
- T. ufvareg1 possesses conserved genomic features within the Tequatrovirus genus.
- The characterized genome and biological properties support T. ufvareg1 as a candidate for food protection against E. coli O157:H7.
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