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Updated: Jul 27, 2025

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
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Molecular Interplay of

Z Muhsen M Ali1, H Ghaith Sachit2, M Jaafar Hussein3

  • 1Faculty of Dentistry, Al-Rafdain University College, Baghdad (00964), Iraq.

Archives of Razi Institute
|June 5, 2023
PubMed
Summary
This summary is machine-generated.

John Cunningham virus (JCV) DNA and Interleukin-1 beta (IL-1β) were detected in Iraqi patients with colorectal cancer. High rates of JCV infection and IL-1β expression suggest their role in colorectal cancer development and progression.

Keywords:
IL1 betaImmunohistochemistryIn situ hybridization

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Area of Science:

  • Oncology
  • Virology
  • Immunology

Background:

  • Colorectal cancer (CRC) is a leading cause of cancer mortality globally.
  • John Cunningham virus (JCV) seroprevalence is high (70-90%) in adults, with recent associations to various malignancies.
  • Interleukin-1β (IL-1β) promotes tumor growth and is a poor prognostic marker in several solid tumors, including colon cancer.

Purpose of the Study:

  • To investigate the presence and concordant expression of JCV DNA and IL-1β in colorectal cancer tissues.
  • To analyze the association between JCV and IL-1β in benign colorectal tumors and healthy controls.
  • To explore the potential role of JCV and IL-1β in the pathogenesis of colorectal adenocarcinoma in an Iraqi cohort.

Main Methods:

  • Tissue samples from 50 colorectal cancer patients, 30 benign colonic tumor patients, and 20 controls were analyzed.
  • JCV DNA detection was performed using chromogenic in situ hybridization (CISH).
  • IL-1β expression was assessed by immunohistochemistry (IHC).

Main Results:

  • JCV DNA was detected in 42% of colorectal carcinoma tissues and 6.7% of benign tumors; no JCV was found in controls.
  • IL-1β positivity was observed in 52% of colorectal carcinomas, 43.3% of benign tumors, and 20% of controls.
  • A high rate of concordant JCV infection and IL-1β expression was noted in colorectal cancer patients.

Conclusions:

  • The study indicates a significant presence of JCV DNA and elevated IL-1β expression in Iraqi patients with colorectal adenocarcinoma.
  • The findings suggest a potential role for JCV and IL-1β in the development and progression of both malignant and benign colorectal tumors.
  • Further research is warranted to elucidate the specific mechanisms underlying the association between JCV, IL-1β, and colorectal cancer.