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Pathological and Molecular detection of

Z Waheed Khudiar Khareeb1, Z Ismail Ibrahim2, F Ali Abdullah3

  • 1Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Basrah, Basrah, Iraq.

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This summary is machine-generated.

Mycoplasma ovipneumonea causes atypical pneumonia in sheep. This study isolated and identified M. ovipneumonea in Iraqi sheep, confirming its prevalence and pathological effects.

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Area of Science:

  • Veterinary Microbiology
  • Animal Pathology

Background:

  • Mycoplasma ovipneumonea is a significant cause of atypical pneumonia in sheep and goats.
  • It is frequently isolated from pneumonic cases but can also reside in healthy animals' respiratory tracts.

Purpose of the Study:

  • To isolate, identify, and conduct a pathological examination of Mycoplasma ovipneumonea in sheep.
  • To investigate the prevalence of M. ovipneumonea in sheep exhibiting respiratory symptoms in Basrah, Iraq.

Main Methods:

  • Bacterial isolation from nasal, tracheal, and bronchial swabs on PPLO agar.
  • DNA extraction for PCR analysis targeting the 16S rRNA gene.
  • Histopathological examination of lung tissues to assess pulmonary changes.

Main Results:

  • Mycoplasma isolates exhibited characteristic "fried egg" colony morphology on PPLO agar.
  • PCR confirmed the presence of Mycoplasma sp. via the 16S rRNA gene.
  • Pathological findings included respiratory congestion, edema, hemorrhagic spots, inflammatory exudate, and acute fibrinous-suppurative broncho-interstitial pneumonia.

Conclusions:

  • Mycoplasma ovipneumoniae is a prevalent respiratory infectious disease in sheep in the Basrah province of Iraq.
  • The study confirmed frequent bacterial isolation and associated pneumonic pathological changes in affected sheep.