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Related Experiment Videos

Method for processing leukocyte- and platelet-poor red cells in closed bags.

T Shimizu, Y Ishikawa, H Tsurumi

    Vox Sanguinis
    |January 1, 1986
    PubMed
    Summary

    A modified warm-centrifuge method efficiently removes 98% of leukocytes and 97% of platelets from red blood cells. This processing allows for extended storage of leukocyte- and platelet-poor red cells, simplifying blood banking operations.

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    Area of Science:

    • Blood banking and transfusion medicine
    • Cellular processing and preservation
    • Hematology

    Background:

    • Efficient processing of leukocyte- and platelet-poor red blood cells is crucial for transfusion safety and logistics, especially during peak demand periods.
    • Current methods for preparing reduced-component blood products can be labor-intensive and may impact red cell viability.
    • The need for readily available, long-term stored red cell units necessitates innovative processing techniques.

    Purpose of the Study:

    • To evaluate a modified warm-centrifuge method for processing leukocyte- and platelet-poor red blood cells in a closed system.
    • To assess the efficiency of leukocyte and platelet removal and the recovery rate of red cells.
    • To analyze the biochemical changes and storage viability of processed red cells.

    Main Methods:

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    • Whole blood was centrifuged in a quadruple bag system to separate red cells.
    • Supernatant plasma was transferred, and red cells were diluted with a phosphate buffer and incubated at 37°C.
    • Red cells were re-centrifuged, and the buffy coat-poor layer was isolated and stored in a preservative solution at 4°C.

    Main Results:

    • The modified warm-centrifuge method removed 98% of leukocytes and 97% of platelets, with an 86% red cell recovery.
    • Biochemical changes in stored red cells were comparable to conventionally processed cells, with a noted rapid decrease in 2,3-diphosphoglycerate.
    • Processed units demonstrated potential for storage exceeding 14 days with minimal cell damage.

    Conclusions:

    • The modified warm-centrifuge method provides a simple and effective means of producing leukocyte- and platelet-poor red cells in a closed system.
    • This processing technique facilitates easier delivery and storage of reduced-component blood products, particularly beneficial for blood centers.
    • The method supports the preparation of red cell units with extended shelf-life and minimal cellular compromise, enhancing transfusion preparedness.