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Study of Some Resistance Genes in Clinical

E Abed Gumar1, A Salim Hamzah2, W Fadhil Hamad1

  • 1Department of Medical Laboratory Techniques, College of Health and Medical Technology, Middle Technical University, Baghdad, Iraq.

Archives of Razi Institute
|June 5, 2023
PubMed

Insights

This study identified antibiotic resistance genes in multidrug-resistant Proteus mirabilis from Iraq. Iraqi strains showed genetic variations compared to global strains, with IntI1 and blaTEM genes being highly prevalent.

Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • Proteus mirabilis is an opportunistic bacterium causing clinical diseases.
  • Multidrug resistance (MDR) in P. mirabilis poses a significant public health threat.

Purpose of the Study:

  • To detect antibiotic resistance genes (blaTEM, aac(6')-Ib, qnrA, IntI2, IntI1) in MDR P. mirabilis clinical isolates from Iraq.
  • To investigate the phylogenetic relationships of these resistance genes among Iraqi and global strains.

Main Methods:

  • Identification of 95 P. mirabilis isolates from 500 clinical samples using VITEK-2 Compact system.
  • Antibiotic susceptibility testing against twelve antibiotics.
  • Gene sequencing and phylogenetic analysis using NCBI database.

Main Results:

  • High prevalence of resistance genes: IntI1 (100%), blaTEM (90%), aac(6')-Ib (80%), IntI2 (80%).
  • Phylogenetic analysis revealed genetic variations in Iraqi isolates compared to global strains.
  • Sequenced genes registered in NCBI with accession numbers.

Conclusions:

  • MDR P. mirabilis in Iraq harbors key antibiotic resistance genes.
  • Genetic variations in resistance genes may influence treatment strategies.
  • Genomic surveillance is crucial for tracking antibiotic resistance patterns.
Keywords:
Antibiotic resistance genesIntegronsIraqi clinical samplesMultidrug Proteus mirabilisNCBI

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