Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Validation of a molecular workflow for Cochliomyia hominivorax (New World screwworm) identification in field samples.

Scientific reports·2026

Same author

Near-complete gapless assembly of <i>Babesia caballi</i> NVSL reference.

Microbiology resource announcements·2026

Same author

Results of the AiM, PLan, and act on LIFestYles (AMPLIFY) Weight Loss Intervention Among Survivors of Obesity-Related Cancers: A Randomized Clinical Trial.

Journal of the National Comprehensive Cancer Network : JNCCN·2026

Same author

Short-Term Impact of Betaine Supplementation on Ruminal Microbial Relative Abundance, Nutrient Digestibility, Serum Metabolites, and Milk Composition in Heat-Stressed Dairy Cows.

Veterinary medicine and science·2026

Same author

Detection of highly pathogenic avian influenza A(H5N1) clade 2.3.4.4b virus in cull dairy cows with underlying respiratory and systemic disease.

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2026

Same author

Metagenomic analysis of the camel rumen archaeome and its functional potential.

Frontiers in veterinary science·2026

Search research articles

Related Experiment Video

Updated: Jul 27, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Whole genome sequencing of

Mohamed Zeineldin¹, Patrick Camp¹, David Farrell¹

¹National Veterinary Services Laboratories, Veterinary Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Ames, IA, United States.

Frontiers in Microbiology

|June 5, 2023

Summary

This summary is machine-generated.

Whole genome sequencing (WGS) of Mycobacterium bovis (M. bovis) is now possible directly from tissue samples, eliminating the need for bacterial culture. This advancement significantly speeds up genomic analysis for research and diagnostics.

Keywords:

Mycobacterium bovis clinical samples diagnostic sequencing target enrichment

More Related Videos

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Related Experiment Videos

Last Updated: Jul 27, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Area of Science:

Genomics
Microbiology
Veterinary Medicine

Background:

Whole genome sequencing (WGS) of Mycobacterium bovis (M. bovis) is crucial for clinical diagnostics and research.
Current methods require culturing M. bovis from tissue, which is time-consuming and challenging due to high host DNA contamination.
Direct DNA sequencing from tissue is hindered by the overwhelming amount of host DNA.

Purpose of the Study:

To evaluate an RNA-based targeted enrichment method for sequencing M. bovis DNA directly from tissue samples without prior culture.
To overcome the challenge of host DNA contamination in direct tissue sample sequencing.
To assess the feasibility and accuracy of direct M. bovis WGS from infected animal tissues.

Main Methods:

Developed and optimized an RNA-based targeted enrichment method using SureSelect custom capture library RNA baits.
Applied the SureSelect XT HS2 target enrichment system for Illumina paired-end sequencing.
Validated the method using spiked tissue samples and naturally/experimentally infected animal tissues with varying M. bovis loads (Ct values).

Main Results:

Achieved high mean genome coverage (99.1% and 98.8%) in spiked samples, demonstrating method sensitivity.
Successfully sequenced M. bovis genomes from infected tissue samples with high mean coverage (99.56%) and variable depth (9.2×–72.1×).
Genomic data (spoligotyping and group assignment) from direct tissue sequencing accurately matched results from cultured isolates.

Conclusions:

Direct WGS of M. bovis DNA from tissue samples using RNA-based targeted enrichment is feasible and accurate.
This method significantly reduces turnaround time compared to culture-based sequencing.
The approach holds potential for routine application in research and diagnostic settings for rapid M. bovis genomic analysis.