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Mapping of the
Anuj Sharma1, Jian Li2,3, Rebecca Wente2,3
1Department of Plant Pathology, University of Florida, Gainesville, FL, United States.
Frontiers in Plant Science
|June 5, 2023
Summary
Researchers mapped two pepper bacterial spot resistance genes, bs5 and bs6, using genotyping-by-sequencing. This work aids marker-assisted selection for disease resistance in pepper breeding programs.
Area of Science:
- Plant Pathology
- Genetics
- Genomics
Background:
- Bacterial spot, caused by Xanthomonas euvesicatoria, significantly impacts pepper (Capsicum annuum L.) production in warm, humid climates.
- Genetically resistant cultivars offer an effective strategy for managing bacterial spot disease.
Purpose of the Study:
- To map the genomic locations of two recessive resistance genes, bs5 and bs6, in pepper.
- To identify candidate genes associated with non-race-specific resistance to bacterial spot.
Main Methods:
- Utilized genotyping-by-sequencing (GBS) for initial mapping of bs5 and bs6 loci.
- Developed segregating populations by crossing susceptible Early CalWonder (ECW) with near-isogenic resistant lines.
- Performed fine-mapping to delimit resistance intervals and identified candidate genes based on sequence polymorphisms.
Main Results:
- The bs5 locus was mapped to a ~535 Kbp interval on chromosome 3.
- The bs6 locus was mapped to a ~666 Kbp interval on chromosome 6.
- Identified 14 candidate genes for bs5 and 8 candidate genes for bs6.
Conclusions:
- This research provides precise locations for bs5 and bs6, facilitating marker-assisted selection in pepper breeding.
- The identified candidate genes are crucial for understanding the molecular basis of bacterial spot resistance in pepper.
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