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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
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Genomic Analysis and Antimicrobial Resistance of
Daitao Zhang1, Xin Zhang1, Bing Lyu1
1Beijing Center for Disease Prevention and Control, Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing, China.
China CDC Weekly
|June 5, 2023
Summary
Campylobacter jejuni strains in Beijing show high genetic diversity and increasing resistance to common antibiotics like ciprofloxacin and tetracycline. This highlights the need for ongoing surveillance of virulence and antimicrobial resistance in this pathogen.
Area of Science:
- Microbiology
- Genomics
- Public Health
Background:
- Campylobacter jejuni is a primary cause of bacterial gastroenteritis globally.
- Understanding its genetic makeup and resistance patterns is crucial for public health.
Purpose of the Study:
- To determine the genetic diversity, virulence factors, and antimicrobial resistance determinants of C. jejuni.
- To analyze strains from a 3-year surveillance period in Beijing, China.
Main Methods:
- Whole genome sequencing (WGS) of 184 clinical C. jejuni isolates.
- Antimicrobial susceptibility testing using agar dilution.
- Multilocus sequence typing (MLST) and phylogenetic analysis.
Main Results:
- High genetic diversity observed with 71 sequence types (STs) and 19 clonal complexes (CCs).
- Significant resistance rates to ciprofloxacin (76.6%), nalidixic acid (76.1%), and tetracycline (71.2%).
- 41.8% of isolates showed multidrug resistance; virulence factors and resistance genes were characterized.
Conclusions:
- C. jejuni in Beijing exhibits diverse genotypes and significant resistance to quinolones and tetracycline.
- The study underscores the importance of monitoring virulence and antimicrobial resistance in C. jejuni.
- Findings provide insights into the evolution and epidemiology of C. jejuni in the region.
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